Summary
A mutant strain devoid of the colicin A protease activity (cpr) located at the external face of the outer membrane has been isolated. The location of the mutation (about 74 min on the genetic map), its pleiotropic effects concerning mainly the protein composition of the outer membrane and sensitivity to phages and colicins, are very similar in cpr and in tpo, envZ, perA strains that are affected in the ompB locus. Conditions resulting in inhibition of the colicin A protease activity also result in transcriptional regulation of OmpF, OmpC, and LamB protein synthesis. The possibility for this protease as an osmosensor of the cell's external environment is discussed.
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Van Alphen W, Lugtenberg B (1977) Influence of the osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli K 12. J Bacteriol 131:623–630
Bassford PJ, Diedrich DL, Schnaitman CA, Reeves P (1977) Outer membrane proteins of Escherichia coli. VI. Protein alteration in bacteriophage resistant mutants. J Bacteriol 131:608–622
Cavard D, Lazdunski C (1979) Purification and molecular properties of a new colicin. Eur J Biochem 96:519–524
Cavard D, Lazdunski C (1981) Involvement of BtuB and OmpF proteins in binding and uptake of colicin A. FEMS Lett 12:311–316
Cavard D, Regnier P, Lazdunski C (1982) Specific cleavage of colicin A by outer membrane proteases from sensitive and insensitive strains of Escherichia coli. FEMS Lett 14:283–287
Dietrich DL, Summers AO, Schnaitman CA (1977) V. Evidence that protein 1 and bacteriophage-directed protein 2 are different polypeptides. J Bacteriol 131:598–607
Hall MN, Silhavy TJ (1979) Transcriptional regulation of Escherichia coli K12 major outer membrane protein 1b. J Bacteriol 140:342–350
Hall MN, Sihavy TJ (1981a) The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12. J Mol Biol 146:23–43
Hall MN, Silhavy TJ (1981b) Genetic analysis of the ompB locus in Escherichia coli K12. J Mol Biol 151:1–15
Kennedy EP (1982) Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli. Proc Natl Acad Sci USA 79:1092–1095
Konisky J (1977) The bacteriocins. In: The Bacteria, vol 7. Academic Press. New York, Chap 2, pp 71–135
Laimins LA, Rhoads DB, Epstein W (1981) Osmotic control of kdp operon expression in Escherichia coli. Proc Natl Acad Sci USA 78:464–468
Lazdunski C, Pages JM, Louvard D (1975) Antibodies as probes for detection of conformational changes in proteins. A model study with the alkaline phosphatase of Escherichia coli. J Mol Biol 97:309–335
Little JW, Mount DW (1982) The SOS regulatory system of Escherichia coli. Cell 29:11–22
Lugtenberg B, Meijers J, Peters R, Van der Hoek P, Van Alphen L (1975) Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bands. FEBS Lett 58:254–258
Lugtenberg B, Peters R, Bernheimer H, Berendsen W (1976) Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Mol Gen Genet 147:251–262
Luria SE, Adams JN, Ting RC (1960) Transduction of lactose-utilizing ability among strains of Escherichia coli and Salmonella dysenteriae and the properties of the transducing phage particles. Virology 12:348–390
Miller JH (1972) In: Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Pages JM, Piovant M, Varenne S, Lazdunski C (1978) Mechanistic aspects of the transfer of nascent periplasmic proteins across the cytoplasmic membrane in Escherichia coli. Eur J Biochem 86:589–602
Pages JM, Lazdunski C (1982) Transcriptional regulation of ompF and lamB genetic expression by local anesthetics. FEMS Lett 15:153–157
Pugsley AP, Conrard DJ, Schnaitman CA, Gregg TI (1980) In vivo effects of local anesthetics on the production of major outer membrane proteins by Escherichia coli. Biochim Biophys Acta 599:1–12
Wandersman C, Moreno F, Schwartz M (1980) Pleiotropic mutations rendering Escherichia coli K12 resistant to phage TP1. J Bacteriol 143:1374–1383
Wanner BL, Sarthy A, Beckwith J (1979) Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins. J Bacteriol 140:229–239
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Cavard, D., Pages, J.M. & Lazdunski, C.J. A protease as a possible sensor of environmental conditions in E. coli outer membrane. Mol Gen Genet 188, 508–512 (1982). https://doi.org/10.1007/BF00330057
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DOI: https://doi.org/10.1007/BF00330057