Summary
An opr24 mutation decreasing the degradation of RNA polymerase ββ′ subunits was found among the Ts+ revertants of Ts strain carring an rpoC1 mutation which stimulates overproduction of these polypeptides. The opr24 mutation is allele-non-specific, for it decreases the degradation of the mutant β and β′ subunits, the amberfragment of the β subunit and the non-mutant β′ subunit. Moreover, opr24 reduces the proteolysis of abnormal proteins containing canavanine.
The opr24 mutation maps between 17 and 21 min on the E. coli genetic map.
The results demonstrate that a slow proteolysis rate in the bacterial cell can supress conditional lethal mutations.
In addition, the slower degradation and the ensuing accumulation of ββ′ subunits does not effect the rate of the ββ′ subunit synthesis.
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Communicated by D.M. Goldfarb
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Sever, I.S., Kalyaeva, E.S., Danilevskaya, O.N. et al. Decreased degradation of ββ′ RNA polymerase subunits and abnormal proteins in a mutant E. coli . Mol Gen Genet 188, 494–498 (1982). https://doi.org/10.1007/BF00330055
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DOI: https://doi.org/10.1007/BF00330055