Summary
The cya gene region of Escherichia coli K12 has been cloned into plasmid pBR322. Detailed analysis of the locus, using in vitro recombination techniques as well as specific labelling of gene products has given information on the organization and products of the region. The cya gene is preceded by two DNA segments which behave as promoters of equivalent strength; a divergent, very strong, promoter is also present in the same control region. The former pair of promoters direct transcription of the cya gene which is expressed as a 95,000 dalton polypeptide; the latter promoter controls synthesis of a 40,000 dalton polypeptide. The cya gene product can complement a cyaΔ mutation when fully expressed or when expressed as a protein truncated at its carboxy terminal end.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
An G, Friesen JD (1979) Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacteriol 140:400–407
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K 12, edition 6. Microbiol Rev 44:1–56
Birnboïm HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7:1513–1523
Botsford JL, Drexler M (1978) The cyclic 3′, 5′ adenosine monophosphate receptor protein and regulation of cyclic 3′, 5′ adenosine monophosphate synthesis in Escherichia coli. Mol Gen Genet 165:47–56
Casadaban MJ, Chou J, Cohen SN (1980) In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol 143:971–980
Clarke L, Carbon J (1976) A clonal bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell 9:91–99
Danchin A, Dondon L, Joseph E, Ullmann A (1981) Transcription-translation coupling and polarity: a possible role of cyclic AMP. Biochimie 63:419–424
Davis DN, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor, New York
Guidi-Rontani C, Danchin A, Ullmann A (1981) Isolation and characterization of a mutant affected in the regulation of adenylate cyclase of Escherichia coli. J Bacteriol 148:753–761
Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197
Joseph E, Bernsley C, Guiso N, Ullmann A (1982) Multiple regulation of the activity of adenylate cyclase in Escherichia coli. Mol Gen Genet 185:262–268
Läemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Majerfeld IH, Miller D, Spitz E, Rickenberg HV (1981) Regulation of the synthesis of adenylate cyclase in Escherichia coli by the cAMP-cAMP receptor protein complex. Mol Gen Genet 181:470–475
Miller JH (1972) In: Experiments in molecular genetics. Cold Spring Harbor, New York
Musso RE, Di Lauro R, Adhya S, De Combrugghe B (1977) Dual control for transcription of galactose operon by cyclic AMP and its receptor protein at two interspersed promoters. Cell 12:847–854
Perlman RL, Pastan I (1969) Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli. Biochem Biophys Res Commun 37:151–157
Roy A, Danchin A (1981) Restriction map of the cya region of the Escherichia coli K 12 chromosome. Biochimie 63:719–722
Sancar A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693
Scott JF, Kornberg A (1978) Purification of the rep protein of Escherichia coli. An ATPase which separates duplex DNA strands in advance of replication. J Biol Chem 253:3292–3297
Stüber D, Bujard H (1981) Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci USA 78:167–171
Sutcliffe JG (1979) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Quant Biol 43:77–90
Thompson R, Hugues SG, Broda P (1974) Plasmid identification using specific endonucleases. Mol Gen Genet 133:145–149
Ullmann A, Danchin A (1982) Role of cyclic AMP in bacteria. Adv Cyclic Nucleotide Res 15 (in press)
Ullmann A, Perrin D (1970) Complementation in β-galactosidase. In: Beckwith JR, Zipser D (eds) The lactose operon. pp. 143–172
Uzan M, Favre R, Gallay E, Caro L (1981) Genetical and structural analysis of a group of λilv and λrho transducing phages. Mol Gen Genet 182:462–479
Wang JYJ, Clegg DO, Koshland E Jr (1981) Molecular cloning and amplification of the adenylate cyclase gene. Proc Natl Acad Sci USA 78:4684–4688
Yang K, Epstein W (1980) Purification of adenylate cyclase of Escherichia coli K 12. Fed Proc 39:2107 (abstract no. 2655)
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Roy, A., Danchin, A. The cya locus of escherichia coli K12: Organization and gene products. Mol Gen Genet 188, 465–471 (1982). https://doi.org/10.1007/BF00330050
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330050