Summary
The cloning and sequence of the regulatory region of the threonine operon of E. coli K12 carried by a constitutive mutant are presented. In this mutant the transcription termination signal, located upstream from the first structural gene, is deleted. These results strongly support the model of attenuation for the regulation of the threonine operon as first proposed by Gardner (1979)
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berkner KL, Folk WR (1977) Polynucleotide kinase exchange reaction. J Biol Chem 252:3176–3184
Berman ML, Fowler A, Zabin I, Christensen L, Fiil NP, Hall MN (submitted) Use of gene fusions for cloning and sequence analysis of the ompF gene of Escherichia coli
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Casadaban M (1976) Transposition and fusion of the lac genes to selected promoters in E. coli using bacteriophage lambda and Mu. J Mol Biol 104:541–555
Casadaban M, Cohen SN (1980) Analysis of gene control signal by DNA susion and cloning in Escherichia coli. J Mol Biol 138:179–207
Cohen GN, Dautry-Varsat A (1980) The aspartokinases-homoserine dehydrogenases of E. coli. In: Bisswanger H, Schmincke-Ott E (eds) Multifunctional proteins, 49–121
Cossart P, Katinka M, Yaniv M, Saint-Girons I, Cohen GN (1979) Construction and expression of a hybrid plasmid containing the E. coli thrA and thrB genes. Mol Gen Genet 175:39–44
Cossart P, Katinka M, Yaniv M (1981) Nucleotide sequence of the thrB gene of E. coli and its two adjacent regions, the thrAB and thrBC junctions. Nucleic Acids Res 9:339–346
Farabaugh PJ, Schmeissner U, Hofer M, Miller JH (1978) Genetic studies of the lac repressor. VII. on the molecular nature of spontaneous hotspots in the lacI gene of E. coli. J Mol Biol 126:847–863
Gardner JF (1979) Regulation of the threonine operon: tandem threonine and isoleucine codons in the control region and translational control of transcription termination. Proc Natl Acad Sci USA 76:1706–1710
Gardner JF (1982) Initiation, pausing and termination of transcription in the threonine operon regulatory region. J Biol Chem 257:3896–3904
Jackson EN, Yanofsky C (1973) The region between the operator and first structural gene of the tryptophan operon of E. coli have a regulatory function. J Mol Biol 76:89–101
Katinka M, Cossart P, Sibilli L, Saint-Girons I, Chalvignac MA, Le Bras G, Cohen GN, Yaniv M (1980) Nucleotide sequence of the thrA gene Proc Natl Acad Sci USA 77:5730–5733
Maniatis T, Jeffrey A, Saude H van de (1975) Chain length determination of small double and single stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14:3787–3794
Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564
McDonnel MW, Simon MN, Studier FW (1976) Analysis of restriction T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol 110:119–146
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Peacock AC, Dingman CW (1967) Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry 6:1818–1827
Saint-Girons I (1978) New regulatory mutations affecting the expression of the threonine operon in E. coli K12. Mol Gen Genet 162:95–100
Saint-Girons I, Margarita D (1978) Fine structure analysis of the threonine operon in E. coli K12. Mol Gen Genet 162:101–107
Sanger F, Coulson AR (1978) The use of thin acrylamide gels for DNA sequencing. FEBS Lett 87:107–110
Tanaka T, Weisblum B (1975) Construction of a colicin E1-R factor. Composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol 121:345–362
Thèse J, Saint-Girons I (1974) Threonine locus of E. coli K12: genetic structure and evidence for an operon. J Bacteriol 118:990–998
Tiollais P, Rambach A, Buc H (1974) Large scale purification of a bacterial gene. FEBS Lett 48:96–100
Yanofsky C (1981) Attenuation in the control of expression of bacterial operons. Nature 289:751–758
Author information
Authors and Affiliations
Additional information
Communicated by G. O'Donovan
Rights and permissions
About this article
Cite this article
Parsot, C., Saint-Girons, I. & Cossart, P. DNA sequence change of a deletion mutation abolishing attenuation control of the threonine operon of E. coli K12. Mol Gen Genet 188, 455–458 (1982). https://doi.org/10.1007/BF00330048
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330048