Summary
In the allele adh1-2F11 of Zea mays the 1.3 kb transposable element Ds2 is inserted in the fourth exon of Adh1. Two major RNAs of 3.0 and 1.6 kb are transcribed from this allele. While the 3.0 kb transcript also contains the Ds2 sequences, the 1.6 kb transcript has lost the Ds2 sequences by an alternative splicing process. In this process, a normal 5′ splice site is joined to a cryptic 3′ splice site located within exon 4. This cryptic 3′ splice site is not used in a wild-type Adh1F allele. Other minor transcripts of adh1-2F11 are prematurely terminated and polyadenylated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aebi M, Hornig H, Padgett RA, Reiser J, Weissmann C (1986) Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA. Cell 47:555–565
Bennetzen JL, Swanson J, Taylor WC, Freeling M (1984) DNA insertion in the first intron of maize Adh1 affects message levels: Cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci USA 81:4125–4128
Brown JWS, Feix G, Frendewey D (1986) Accurate in vitro splicing of two pre-mRNA plant introns in a HeLa cell nuclear extract. EMBO 5:2749–2758
Döring HP, Freeling M, Hake S, Johns MA, Kunze R, Merckelbach A, Salamini F, Starlinger P (1984) A Ds-mutation of the Adh1 gene in Zea mays L. Mol Gen Genet 193:199–204
Kühne T, Wieringa B, Reiser J, Weissmann C (1983) Evidence against a scanning model of RNA splicing. EMBO J 2:727–733
Lang KM, Spritz RA (1983) RNA splice site selection: Evidence for a 5′ to 3′ scanning model. Science 220:1351–1355
Leff SE, Rosenfeld MG, Evans RM (1986) Complex transcriptional units: diversity in gene expression by alternative RNA processing. Annu Rev Biochem 55:1091–1117
Merckelbach A, Döring HP, Starlinger P (1986) The aberrant Ds element in the adh1-2F11:Ds2 allele. Maydica 31:109–122
Mount SM (1982) A catalogue of splice junctions. Nucleic Acids Res 10:459–472
Padgett RA, Grabowski PJ, Konarska M, Seiler S, Sharp PA (1986) Splicing of messenger RNA precursors. Annu Rev Biochem 55:1119–1150
Peacock WJ, Dennis ES, Gerlach WL, Sachs MM, Schwartz D (1984) Insertion and excision of Ds controlling elements in maize. Cold Spring Harbor Symp Quant Biol 49:347–354
Reed R, Maniatis T (1986) A role for exon sequences and splice-site proximity in splice-site selection. Cell 46:681–690
Sachs MM, Dennis ES, Gerlach WL, Peacock WJ (1986) Two alleles of maize alcohol dehydrogenase I have 3′ structural and poly(A) addition polymorphisms. Genetics 113:449–467
Sharp P (1981) Speculations on RNA splicing. Cell 23:643–646
Solnick D (1985) Alternative splicing caused by RNA secondary structure. Cell 43:667–676
Author information
Authors and Affiliations
Additional information
Communicated by H. Saedler
Rights and permissions
About this article
Cite this article
Simon, R., Starlinger, P. Transposable element Ds2 of Zea mays influences polyadenylation and splice site selection. Mol Gen Genet 209, 198–199 (1987). https://doi.org/10.1007/BF00329859
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00329859