Summary
Using field-inversion gel electrophoresis we defined an electrophoretic karyotype for the yeast, Candida albicans. The karyotype is distinct from other species of Candida and is species specific. A total of five distinct chromosomal mobility groups were observed, at least four of which are composed of a minimum of two fragments each. From the apparent sizes of these fragments relative to the large chromosomes of the morphologically related yeast Saccharomyces cerevisiae, together with the known genome size of this organism, we conclude that the karyotype is the result of the migration of intact chromosomes.
Similar content being viewed by others
References
Auger P, Dumas C, Joly J (1979) A study of 666 strains of Candida albicans: correlation between serotype and susceptibility to 5-fluorocytosine. J Infect Dis 139: 590–594
Botstein D, Falco SC, Stuart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17–24
Carle G, Olson M (1984) Separation of chromosomal DNA molecules by orthogonal-field-alteration gel electrophoresis. Nucleic Acids Res 12: 5647–5664
Carle G, Olson M (1985) An electrophoretic karyotype for yeast. Proc Natl Acad Sci USA 82: 3756–3760
Carle G, Frank M, Olson M (1986) Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science 232: 65–68
deRepentigny L, Kuykendall R, Chandler F, Broderson J, Reiss E (1984) Comparison of serum mannan, arabinitol, and mannose in experimental disseminated candidiasis. J Clin Microbiol 19: 804–812
Hilton C, Markie D, Corner B, Rikkerink E, Poulter, R (1985) Heat shock induces chromosome loss in the yeast Candida albicans. Mol Gen Genet 200: 162–168
Hughes J, Culver J, White W, Jarvis W, Munn V, Mosser J, Emori T (1983) Nosocomial infection surveillance, 1980–1982. Morbid Mortal Weekly Rep 32: 1–16
Hurley D, Fauci A (1975). Disseminated candidiasis. I. An experimental model in the guinea pig. J Infect Dis 131: 516–521
Lauer G, Roberts T, Klotz L (1977) Determination of the nuclear DNA content of Saccharomyces cerevisiae and implications for the organization of DNA in yeast chromosomes. J Mol Biol 114: 507–526
Lehmann P, Reiss E (1980) Comparison by ELISA of serum anti-Candida albicans mannan IgG levels of a normal population and in diseased patients. Mycopathologia 70: 89–93
Mortimer R, Schild D (1980) Genetic map of Saccharomyces cerevisiae. Microbiol Rev 44: 519–571
Myerowitz R, Pazin G, Allen C (1977) Disseminated candidiasis: changes in incidence, underlying diseases, and pathology. Am J Clin Pathol 68: 29–38
Reiss E, Stone S, Hasenclever H (1974) Serological and cellular immune activity of peptidoglucomannan fractions of Candida albicans cell walls. Infect Immun 9: 881–890
Rigby P, Dieckman M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with polymerase I. J Mol Biol 113: 237–251
Riggsby W, Torres-Bauza L, Wills J, Townes T (1982) DNA content, kinetic complexity, and the ploidy question in Candida albicans. Mol Cell Biol 2: 853–862
Rüchel R (1981) Properties of a purified proteinase from the yeast Candida albicans. Biochim Biophys Acta 659: 99–113
Schwartz D, Cantor C (1984) Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37: 67–75
Snell R, Wilkins R (1986) Separation of chromosomal DNA molecules from C. albicans by pulsed field gel electrophoresis. Nucleic Acids Res 14: 4401–4406
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–518
Whelan W, Markie D, Simpkin K, Poulter R (1985) Instability of Candida albicans hybrids. J Bacteriol 161: 1131–1136
Wills J, Lasker B, Sirotkin K, Riggsby W (1984) Repetitive DNA of Candida albicans: nuclear and mitochondrial components. J Bacteriol 157: 918–924
Author information
Authors and Affiliations
Additional information
Communicated by C.P. Hollenberg
Rights and permissions
About this article
Cite this article
Lott, T.J., Boiron, P. & Reiss, E. An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples. Mol Gen Genet 209, 170–174 (1987). https://doi.org/10.1007/BF00329854
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00329854