Summary
Deletion mutations, 3–19 amino acids in size, were introduced into the transit peptide (57 amino acids) of a small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase from pea. Transport of the authentic small subunit precursor (pSSU) and of the mutant pSSUs by isolated chloroplasts of pea was examined. We show that the transit peptide contains two different, separated functional regions. A deletion mutation in the central region of the transit peptide, a region purported to be important for function, barely affected transport. Changes in the amino-terminal region of the transit peptide drastically reduced transport. Processing of mutants affected in either the amino-terminal or central portion of the transit peptide appeared normal. A deletion mutation at the carboxy-terminus of the transit peptide interfered with both transport and processing. From the aberrant processing we suggest that pSSU is matured in more than one step, and that the maturation signal is located within the carboxy-terminal 16 amino acids. The methionine residue at the evolutionarily conserved cleavage site (cysteine-methionine) between the transit peptide and the mature protein is not essential for processing.
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Communicated by R. Herrmann
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Reiss, B., Wasmann, C.C. & Bohnert, H.J. Regions in the transit peptide of SSU essential for transport into chloroplasts. Mol Gen Genet 209, 116–121 (1987). https://doi.org/10.1007/BF00329845
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DOI: https://doi.org/10.1007/BF00329845