Summary
The mitochondrial genome of Petunia hybrida line 3704 has been found to contain two transcribed genes for the proteolipid subunit of the ATP synthase complex (atp 9). The 5′ and 3′ flanking sequences of the second atp 9 gene differ from those of another atp 9 gene previously sequenced by Young et al. (1986), while the coding region exhibits only one silent base change. The transcript termini of the two divergent atp 9 genes map to different locations, most of which are surrounded by sequences homologous to putative mitochondrial transcription signals (Hiesel and Brennicke 1985; Schuster et al. 1986; Young et al. 1986). Because transcripts from the atp 9 genes differ in abundance, divergence in the flanking regions of these two genes must affect regulatory elements which control either transcription rate or transcript stability.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bland MM, Leavings III CS, Matzinger DF (1986) The tobacco mitochondrial ATPase subunit 9 gene is closely linked to an open reading frame for a ribosomal protein. Mol Gen Genet 204:8–16
Boeshore ML, Hanson MR, Izhar S (1985) A variant mitochondrial DNA arrangement specific to Petunia stable sterile somatic hybrids. Plant Mol Biol 4:125–132
Bonen L, Boer PH, Gray MW (1984) The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J 3:2531–2536
Dawson AJ, Jones VP, Leaver CJ (1984) The apocytochrome b gene in maize mitochondria does not contain introns and is proceeded by a potential ribosome binding site. EMBO J 3:2107–2113
Dawson AJ, Hodge TP, Isaac PG, Leaver CJ, Lonsdale DM (1986) Location of the genes for cytochrome oxidase subunits I and II, apocytochrome b, α-subunit of the F1 ATPase and the ribosomal RNA genes on the mitochondrial genome of maize (Zea mays L.). Curr Genet 10:561–564
Dewey RE, Schuster AM, Levings III CS, Timothy DH (1985) Nucleotide sequence of F0-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci USA 82:1015–1019
Falconet D, Lejeune B, Quetier F, Gray MW (1984) Evidence for homologous recombination between repeated sequences containing 18S and 5S ribosomal RNA genes in wheat mitochondrial DNA. EMBO J 3:297–302
Falconet D, Delorme S, Lejeune B, Sevignac M, Delcher E, Bazetoux S, Quetier F (1985) Wheat mitochondrial 26S ribosomal RNA gene has no intron and is present in multiple copies arising by recombination. Curr Genet 9:169–174
Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94:213–267
Hiesel R, Brennicke A (1985) Overlapping reading frames in Oenothera mitochondria. FEBS Lett 193:164–168
Hiesel R, Schobel W, Schuster W, Brennicke A (1987) The cytochrome oxidase subunit I and subunit III genes in Oenothera mitochondria are transcribed from identical promoter sequences. EMBO J 6:29–34
Isaac PG, Brennicke A, Dunbar SM, Leaver CJ (1985a) The mitochondrial genome of fertile maize (Zea mays L.) contains two copies of the gene encoding the α-subunit of the F1-ATPase. Curr Genet 10:321–328
Isaac PG, Jones VP, Leaver CJ (1985b) The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male sterile plants. EMBO J 4:1617–1623
Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560
Moon E, Kao T-H, Wu R (1985) Pea cytochrome oxidase subunit II has no intron and generates two mRNA transcripts with different 5′ termini. Nucleic Acids Res 13:3195–3212
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Rothenberg M, Hanson MR (1987) Recombination between parental mitochondrial DNA following protoplast fusion can occur in a region which normally does not undergo intragenomic recombination in parental plants. Curr Genet (in press)
Salts Y, Beckmann J (1981) Chloroplast DNA preparation for Petunia and Nicotiana. Plant Mol Biol Newslett 2:73–74
Schuster W, Brennicke A (1986) Pseudocopies of the ATPase α-subunit gene in Oenothera mitochondria are present on different circular molecules. Mol Gen Genet 204:29–35
Schuster W, Hiesel R, Isaac PG, Leaver CJ, Brennicke A (1986) Transcript termini of messenger RNAs in higher plant mitochondria. Nucleic Acids Res 14:5943–5954
Stern DB, Newton KJ (1986) Isolation of plant mitochondrial RNA. Methods Enzymol 118:488–496
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell (in press)
Young EG, Hanson MR, Dierks PM (1986) Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit. Nucleic Acids Res 14:7995–8006
Author information
Authors and Affiliations
Additional information
Communicated by R. Herrmann
Rights and permissions
About this article
Cite this article
Rothenberg, M., Hanson, M.R. Different transcript abundance of two divergent ATP synthase subunit 9 genes in the mitochondrial genome of Petunia hybrida . Mol Gen Genet 209, 21–27 (1987). https://doi.org/10.1007/BF00329831
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00329831