Skip to main content
SpringerLink
Log in

Molecular cloning of Bacillus subtilis genes involved in DNA metabolism

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A λ recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a λ Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anagnostopoulos C, Spizizen J (1961) Requirement for transformation in Bacillus subtilis. J Bacteriol 81:741–746

    Google Scholar 

  • Bachmann BJ (1983) Linkage map of Escherichia coli K-12, Edition 7. Microbiol Rev 47:180–230

    Google Scholar 

  • Bazill GW, Karamata D (1972) Temperature-sensitive mutants of Bacillus subtilis defective in deoxyribonucleotide synthesis. Mol Gen Genet 117:19–29

    Google Scholar 

  • Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523

    Google Scholar 

  • Bochner BR, Huang HC, Schieven GL, Ames BN (1980) Positive selection for loss of tetracycline resistance. J Bacteriol 143:926–933

    Google Scholar 

  • Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472

    Google Scholar 

  • Cohen SN, Chang ACY, Boyer HW, Helling RB (1973) Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci USA 70:3240–3244

    Google Scholar 

  • Dagert M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28

    Google Scholar 

  • Davis RW, Botstein D, Roth JH (1980) (eds) Advanced bacterial genetics: a manual for genetic engineering. Cold Spring Harbor Laboratory, NY, pp 109–111

    Google Scholar 

  • De Vos WM, De Vries STC, Venema G (1983) Cloning and expression of the Escherichia coli recA gene in Bacillus subtilis. Gene 25:301–308

    Google Scholar 

  • Dubnau D (1982) Genetic transformation in Bacillus subtilis. In: Dubnau D (ed) The molecular biology of the bacilli, vol 1. Academic Press, New York, pp 147–177

    Google Scholar 

  • Ferrari E, Henner DJ, Hoch JA (1981) Isolation of Bacillus subtilis genes from a Charon 4 A library. J Bacteriol 146:430–432

    Google Scholar 

  • Ferrari FA, Nguyen A, Lang LD, Hoch JA (1983) Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol 154:1513–1515

    Google Scholar 

  • Ganesan AT, Yehle CO, Yu CC (1973) DNA replication in a polymerase I deficient mutant and the identification of DNA polymerases II and III in Bacillus subtilis. Biochem Biophys Res Commun 50:155–163

    Google Scholar 

  • Gass KB, Cozzarelli NR (1973) Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases. J Biol Chem 248:7688–7700

    Google Scholar 

  • Hanawalt PC, Cooper PK, Ganesan AK, Smith CA (1979) DNA repair in bacteria and mammalian cells. Annu Rev Biochem 48:783–836

    Google Scholar 

  • Karamata D, Gross JD (1970) Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Mol Gen Genet 108:277–287

    Google Scholar 

  • Imada S, Carrol LE, Sueoka N (1980) Genetic mapping of a group of temperature-sensitive dna initiation mutants in Bacillus subtilis. Genetics 94:809–823

    Google Scholar 

  • Lampe MF, Bott KF (1985a) Cloning of the Bacillus subtilis recF gene. Gene 38:139–144

    Google Scholar 

  • Lampe MF, Bott KF (1985b) Genetic and physical organization of the cloned gyrA and gyrB genes of Bacillus subtilis. J Bacteriol 162:78–84

    Google Scholar 

  • Laurent S, Vannier F (1973) Temperature-sensitive initiation of chromosome replication in a mutant of B. subtilis. J Bacteriol 117:329–336

    Google Scholar 

  • Leder P, Tiemeier D, Enquist L (1977) EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the gtWES system. Science 195:175–177

    Google Scholar 

  • Love E, D'Ambrosio J, Brown NC, Dubnau D (1976) Mapping of the gene specifying DNA polymerase III of Bacillus subtilis. Mol Gen Genet 144:313–321

    Google Scholar 

  • Mazza G (1982) Bacillus subtilis rec-assay test with isogenic strains. Appl Environ Microbiol 43:177–184

    Google Scholar 

  • Mazza G, Galizzi A (1978) The genetics of DNA replication, repair and recombination in Bacillus subtilis. Microbiologica 1:111–135

    Google Scholar 

  • Moriya S, Ogasawara N, Yoshikawa H (1985) Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region. Nucleic Acids Res 13:2251–2265

    Google Scholar 

  • Ogasawara N, Seiki M, Yoshikawa H (1983) Replication origin region of Bacillus subtilis chromosome contains two rRNA operons. J Bacteriol 154:50–57

    Google Scholar 

  • Ogasawara N, Moriva S, von Meyenburg K, Hansen FG, Yoshikawa H (1985a) Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli. EMBO J 4:3345–3350

    Google Scholar 

  • Ogasawara N, Moriya S, Yoshikawa H (1985b) Structure and function of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames. Nucleic Acids Res 13:2267–2279

    Google Scholar 

  • Ogasawara N, Moriya S, Mazza G, Yoshikawa H (1986a) A Bacillus subtilis dnaG mutant harbours a mutation in a gene homologous to the dnaN gene of Escherichia coli. Gene 45:227–231

    Google Scholar 

  • Ogasawara N, Moriya S, Mazza PG, Yoshikawa H (1986b) Nucleotide sequence and organization of dna B gene and neighbouring genes on the Bacillus subtilis chromosome. Nucleic Acids Res 14:9989–9999

    Google Scholar 

  • Ott RW, Barnes MH, Brown NC, Ganesan AT (1986) Cloning and characterization of the polC region of Bacillus subtilis. J Bacteriol 165:951–957

    Google Scholar 

  • Piggot PJ, Hoch JA (1985) Revised genetic linkage map of Bacillus subtilis. Microbiol Rev 49:158–179

    Google Scholar 

  • Ratcliff SW, Luh J, Ganesan AT, Behrens B, Thompson R, Montenegro MA, Morelli G, Trautner TA (1979) The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments. Mol Gen Genet 168:165–172

    Google Scholar 

  • Riva S, Van Sluis C, Mastromei G, Attolini C, Mazza G, Polsinelli M, Falaschi A (1975) A new mutant of B. subtilis altered in the initiation of chromosome replication. Mol Gen Genet 137:185–202

    Google Scholar 

  • Spizizen J (1958) Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc Natl Acad Sci USA 44:1072–1078

    Google Scholar 

  • Wang LF, Price CW, Doi RH (1985) Bacillus subtilis dna E encodes a protein homologous to DNA primase of Escherichia coli. J Biol Chem 260:3368–3372

    Google Scholar 

  • Winston S, Sueoka N (1982) DNA replication in Bacillus subtilis. In: Dubnau DA (ed) The molecular biology of the bacilli, vol 1. Academic Press, New York, pp 35–69

    Google Scholar 

  • Zeigler DR, Dean DH (1985) Revised genetic map of Bacillus subtilis 168. FEMS Microbiol Rev 32:101–134

    Google Scholar 

Download references

Author information

Author notes

  1. Marta Perego

    Present address: Scripps Clinic and Research Foundation, 92037, La Jolla, CA, USA

  2. Eugenio Ferrari

    Present address: Genencor, Inc., 94080, South San Francisco, CA, USA

  3. Piergiorgio Mazza

    Present address: Istituto di Tecnica Farmaceutica, Università di Parma, Via M. D'Azeglio 85, I-43100, Parma, Italy

Authors and Affiliations

  1. Dipartimento di Genetica e Microbiologia “A. Buzzati-Traverso”, Università di Pavia, I-27100, Pavia, Italy

    Marta Perego, Eugenio Ferrari, Maria Teresa Bassi, Alessandro Galizzi & Piergiorgio Mazza

Authors
  1. Marta Perego
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eugenio Ferrari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maria Teresa Bassi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alessandro Galizzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Piergiorgio Mazza
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J.W. Lengeler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Perego, M., Ferrari, E., Bassi, M.T. et al. Molecular cloning of Bacillus subtilis genes involved in DNA metabolism. Mol Gen Genet 209, 8–14 (1987). https://doi.org/10.1007/BF00329829

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00329829

Key words

Search

Navigation