Summary
Three mutants of Salmonella typhimurium LT2, which required either pantothenate or β-alanine for growth, were obtained after treatment with N-methyl-N′-nitro-N-nitrosoguanidine. Their phenotype was: SM30 Pan-, SM31 Pan- Met-, SM32 Pan- Thi- (requirement for the thiazole-moiety of thiamine). Neither aspartate, dihydrouracil, nor β-ureidopropionate replaced β-alanine as growth factor. By conjugation it was found that the three genetic lesions (Pan-, Met-, Thi-) were located at about minute 128 of the bacterial chromosome. By transduction 63% linkage was found between the Pan and Met loci, and 84% between the Thi and Pan loci. Probably the thiazole auxotrophy was due to a lesion in the thiG locus. The new genetic locus responsible for the synthesis of β-alanine was named panD.
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Ortega, M.V., Cárdenas, A. & Ubiera, D. panD, a new chromosomal locus of Salmonella typhimurium for the biosynthesis of β-alanine. Molec. gen. Genet. 140, 159–164 (1975). https://doi.org/10.1007/BF00329783
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DOI: https://doi.org/10.1007/BF00329783