Summary
The Escherichia coli HU-2 gene was cloned using a DNA fragment from the HU-1 gene as a probe. The amino acid sequence of the HU-2 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream of the translation initiation codon (AUG) and a possible rhoindependent terminater site downstream of the termination codon (UAA) of the gene.
References
Broyles SS, Pettijohn DE (1986) Interaction of the Escherichia coli HU protein with DNA: Evidence for formation of nucleosomelike structures with altered DNA helical pitch. J Mol Biol 187:47–60
Champoux JI (1978) Proteins that affect DNA conformation. Annu Rev Biochem 47:449–479
Craigie R, Arndt-Jovin DJ, Mizuuchi K (1985) A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition: Protein and DNA substrate requirements. Proc Natl Acad Sci USA 82:7570–7574
Dixon NE, Kornberg A (1984) Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli. Proc Natl Acad Sci USA 81:424–428
Griffith JD (1976) Visualization of prokaryotic DNA in a regularly condensed chromatin-like fiber. Proc Natl Acad Sci USA 73:563–567
Hattori M, Sakaki Y (1986) Dideoxy sequencing method using denatured plasmid templates. Anal Biochem 152:232–238
Ikemura T, Ozeki H (1983) Codon usage and transfer RNA contents: Organism-specific codon-choice patterns in reference to the isoacceptor contents. Cold Spring Harbor Symp Quant Biol 47:1087–1097
Johnson RC, Bruist MF, Simon MI (1986) Host protein requirements for in vitro site-specific DNA inversion. Cell 46:531–539
Kano Y, Yoshino S, Wada M, Yokoyama K, Nobuhara M, Imamoto F (1985) Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli. Mol Gen Genet 201:360–362
Kano Y, Wada M, Nagase T, Imamoto F (1986) Genetic characterization of the gene hupB encoding the HU-1 protein of Escherichia coli. Gene 45:37–44
Kornberg T, Lockwood A, Worcel A (1974) Replication of the Escherichia coli chromosome with a soluble enzyme system. Proc Natl Acad Sci USA 71:3189–3193
Laine B, Kmiecik D, Sautiere P, Biserte G, Cohen-Solal M (1980) Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli. Eur J Biochem 103:447–461
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, USA
Mende L, Timm B, Subramanian AR (1978) Primary structures of two homologous ribosome-associated DNA-binding proteins of Escherichia coli. FEBS Lett 96:395–398
Pribnow D (1975) Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72:784–788
Rouvière-Yaniv J (1978) Localization of HU protein on the Escherichia coli nucleoid. Cold Spring Harbor Symp Quant Biol 42;439–447
Rouvière-Yaniv J, Gros F (1975) Characterization of a novel, low molecular weight DNA binding protein from Escherichia coli. Proc Natl Acad Sci USA 72:3428–3432
Rouvière-Yaniv J, Kjeldgaard NO (1979) Native Escherichia coli HU protein is a heterotypic dimer. FEBS Lett 106:297–300
Rouvière-Yaniv J, Yaniv M, Germond JE (1979) E. coli DNA binding protein HU forms nucleosome-like structure with circular double-stranded DNA. Cell 17:265–274
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding site. Proc Natl Acad Sci USA 71:1342–1346
Stonington OG, Pettijohn DE (1971) The folded genome of Escherichia coli isolated in a protein-DNA complex. Proc Natl Acad Sci USA 68:6–9
Takanami M, Sugimoto K, Sugisaki H, Okamoto T (1976) Sequence of promoter for coat protein gene of bacteriophage fd. Nature 260:297–302
Varshavsky AJ, Nedospasov SA, Bakayev VV, Bakayeva TG, Georgiv GP (1977) Histone-like proteins in the purified Escherichia coli deoxyribonucleoproteins. Nucleic Acids Res 4:2724–2745
Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Worcel A, Burgi E (1972) On the structure of the folded chromosome of Escherichia coli. J Mol Biol 71:127–142
Yamazaki K, Nagata A, Kano Y, Imamoto F (1984) Isolation and characterization of nucleoid proteins from Escherichia coli. Mol Gen Genet 196:217–224
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Communicated by M. Takanami
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Kano, Y., Osato, K., Wada, M. et al. Cloning and sequencing of the HU-2 gene of Escherichia coli . Molec Gen Genet 209, 408–410 (1987). https://doi.org/10.1007/BF00329674
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DOI: https://doi.org/10.1007/BF00329674