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Molecular genetic analysis of the pyr-4 gene of Neurospora crassa

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Summary

By means of S1 nuclease mapping, one transcription origin and three termini are identified for the pyr-4 gene of Neurospora crassa, the same origin being used also by Escherichia coli on the cloned gene. Translation of the clone in mini-cells gives a 50000 dalton gene product, the same size as that determined for the Neurospora native enzyme. Putative CAAT and TATA boxes, and upstream and downstream potential secondary structures, are identified.

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Authors and Affiliations

  1. Department of Genetics, The University of Leeds, LS2 9JT, Leeds, UK

    J. A. Glazebrook, K. Mitchell & A. Radford

Authors
  1. J. A. Glazebrook
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  2. K. Mitchell
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  3. A. Radford
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Communicated by W. Gajewski

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Glazebrook, J.A., Mitchell, K. & Radford, A. Molecular genetic analysis of the pyr-4 gene of Neurospora crassa . Molec Gen Genet 209, 399–402 (1987). https://doi.org/10.1007/BF00329672

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  • DOI: https://doi.org/10.1007/BF00329672

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