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Banding pattern analysis of human chromosomes by use of a urea treatment technique

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Abstract

A new technique to reveal the banding pattern of human chromosomes is described. Slides prepared by the routine air drying technique were treated with urea-Sörensen buffer solution for ten minutes at pH 6.8 at 37° C. Individual pairs of all human chromosomes exhibited a characteristic banding pattern by this technique, and by its use the karyotypes were analysed. With the exception of some minor differences the banding patterns obtained by the present technique appeared to be identical with those obtained by the Giemsa staining and quinacrine fluorescence methods carried out by previous workers.

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Kanazawa University, Kanazawa

    Yukimasa Shiraishi & Tosihide H. Yosida

  2. Department of Cytogenetics, National Institute of Genetics, Misima

    Yukimasa Shiraishi & Tosihide H. Yosida

Authors
  1. Yukimasa Shiraishi
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  2. Tosihide H. Yosida
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Additional information

Contribution No. 877 from the National Institute of Genetics, Japan. Supported by grant-in-aid No. 92332 from the Ministry of Education of Japan.

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Shiraishi, Y., Yosida, T.H. Banding pattern analysis of human chromosomes by use of a urea treatment technique. Chromosoma 37, 75–83 (1972). https://doi.org/10.1007/BF00329559

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  • DOI: https://doi.org/10.1007/BF00329559

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