Summary
We have developed a method for the dissociation and purification of myosatellite cells from white epaxial muscle of carp. The dissociated myosatellite cells were identified by their morphology, their ultrastructure, the formation of multinucleated myotubes containing myofibrils and the immunocytochemical demonstration of desmin. Desmin and 5-bromo-2′-deoxyuridine (BrdU) were used to identify terminally differentiated and proliferating myosatellite cells, respectively. The in vitro behavior of myosatellite cells dissociated from carp of 5 cm standard length differed from that described for myosatellite cells of mammals and birds. No substantial proliferation of the myosatellite cells could be observed. Most cells were differentiated (desmin-positive, BrdU-negative) 17 h after plating, regardless of the medium used. This indicates that the investigated white epaxial muscle of carp of 5 cm standard length contains subpopulations of myosatellite cells, arrested at various stages of differentiation.
Similar content being viewed by others
References
Akster HA (1983) A comparative study of fibre type characteristics and terminal innervation in head and axial muscle of the carp (Cyprinus carpio L): a histochemical and electron-microscopical study. Neth J Zool 33: 164–188
Allen RE (1987) Muscle cell culture as a tool in animal growth research. Fed Proc 46: 290–294
Anderson TF (1951) Techniques for the preservation of three-dimensional structures in preparing specimens for the electron microscope. Trans NY Acad Sci 13: 130–134
Bayne CJ (1986) Pronephric leucocytes of Cyprinus carpio: isolation, separation and characterization. Vet Immunol Immunopathol 12:141–151
Bischoff R (1986) A satellite cell mitogen from crushed adult muscle. Dev Biol 115: 140–147
Bischoff R, Holzer H (1970) Inhibition of myoblast fusion after one round of DNA synthesis in 5-bromodeoxyuridine. J Cell Biol 44: 134–150
Campion DR (1984) The muscle satellite cell; a review. Int Rev Cytol 87: 225–251
Darnell J, Lodish H, Baltimore D (eds) (1986) The cytoskeleton and cellular movements: microtubules. In: Molecular cell biology, Scientific American Books, New York, pp 771–813
Foster RF, Thompson JM, Kaufman SJ (1987) A laminin substrate promotes myogenesis in rat skeletal muscle cultures; analysis of replication and development using anti-desmin and anti BrdUrd monoclonal antibodies. Dev Biol 122: 11–20
Goldspink G (1972) Postembryonic growth and differentiation of striated muscle. In: Bourne GH (ed) The structure and function of muscle, vol 1, 2nd edn., Academic Press, New York, p 179
Goldspink G (1974) Development of muscle. In: Goldspink G (ed) Differentiation and growth of cells in vertebrate tissues Chapman and Hall, London
Greer-Walker M (1970) Growth and development of the skeletal muscle fibers of the cod (Gadus morhua L.) J Cons Int Explor Mer 33: 228–244
Grounds MD, McGeachie JK (1989) Myogenic cells of regenerating adult chicken muscle can fuse into myotubes after a single cell division in vivo. Exp Cell Res 180: 429–439
Inestrosa NC (1982) Differentiation of skeletal muscle cells in culture. Cell Struct Funct 7:91–109
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27: 137a-138a
Kaufman SJ, Foster RF (1985) Remodelling of the myoblast membrane accompanies development. Dev Biol 110:1–14
Kaufmann SJ, Foster RF (1988) Replicating myoblasts express a muscle-specific phenotype. Proc Natl Acad Sci USA 85: 9606–9610
Konigsberg IR (1963) Clonal analysis of myogenesis. Science 140: 1273–1284
Kryvi H (1975) The structure of the myosatellite cells in axial muscles of the shark Galeus melastomus. Anat Embryol 147: 35–44
Kryvi H, Eide A (1977) Morphometric and autoradiographic studies on the growth of red and white axial muscle fibers in the shark Etmopterus spinax. Anal Embryol 151: 17–28
Kühl U, Öcalan M, Timpl R, van der Mark K (1986) Role of laminin and fibronectin in selecting myogenic versus fibrogenic cells from skeletal muscle cells in vitro. Dev Biol 117: 628–636
Lazarides E (1982) Intermediate filaments; a chemically heterogeneous. developmentally regulated class of proteins. Ann Rev Biochem 51: 219–250
Lipton BH (1977) A line-structural analysis of normal and modulated cells in myogenic cultures. Dev Biol 60: 26–47
Mauro A (1961) Satellite cells of skeletal muscle fibers. J Biophys Biochem Cytol 9: 493–495
Mulvaney DR, Marple DN, Merkel RA (1988) Proliferation of skeletal muscle satellite cells after castration and administration of testosterone propionate. Proc Soc Exp Biol Med 188: 40–45
Neff NT, Lowrey C, Decker C, Tovar A, Damsky C, Horwitz AF (1982) A monoclonal antibody detaches embryonic skeletal muscle from extracellular matrices. J Cell Biol 95: 654–666
Öcalan M, Goodman SL, Kühl U, Hauschka SD, von der Mark K (1988) Laminin alters cell shape and stimulates motility and proliferation of murine skeletal myoblasts. Dev Biol 125: 158–167
Ontell M (1974) Muscle satellite cells; a validated technique for light microscopic identification and a quantitative study of changes in their population following denervation. Anat Rec 178: 211–228
Powell RL, Dodson MV, Cloud JG (1989) Cultivation and differentiation of satellite cells from skeletal muscle of the rainbow trout Salmo gairdneri. J Exp Zool 250: 333–338
Quinn LS, Nameroff M, Holtzer H (1984) Age dependent changes in myogenic precursor cell compartment sizes. Evidence for the existence of a stem cell. Exp Cell Res 154: 65–82
Quinn LS, Holtzer H, Nameroff M (1985) Generation of chick skeletal muscle cells in groups of 16 from stem cells. Nature 313: 692–694
Ramaekers FCS, Kant A, Verheyen RHM, Vooys GP, Moesher O, Herman CJ (1983) Mesodermal mixed tumor; diagnosis by analysis of intermediate filament proteins. Am J Surg Pathol: 381–385
Rong PM, Ziller C, Pena-Melian A, Le Douarin NM (1987) A monoclonal antibody specific for avian early myogenic cells and differentiated muscle. Dev Biol 122: 338–353
Sanger JW (1974) The use of cytochalasin B to distinguish myoblasts from fibroblasts in cultures of developing chick striated muscle. Proc Natl Acad Sci USA 71: 3621–3625
Stickland NC (1983) Growth and development of muscle fibers in the rainbow trout (Salmo gairdneri). J Anat 137: 323–333
Takahama H, Mizuhira V, Sasaki F, Watanabe K (1984) Satellite cells in the tail muscles of the urodelan larvae during development. Cell Tissue Res 236: 431–438
Wakshull E, Kahn Bayne E, Chiquet M, Fambrough D (1983) Characterization of a plasma membrane glycoprotein common to myoblasts, skeletal muscle satellite cells, and glia. Dev Biol 100: 464–477
Walsh FS, Moore SE, Woodroofe MN, Hurko O, Nayak R, Brown SM, Dickson JG (1984) Characterisation of human muscle differentiation antigens. Expl Biol Med 9: 50–56
Weatherly AH, Gill HS (1984) Growth dynamics of white myotomal muscle fibres in the bluntnose minnow Pimephales notatus Rafinesque and comparison with rainbow trout Salmo gairdneri Richardson J Fish Biol 25: 13–24
Willemse JJ, van den Berg PG (1978) Growth of striated muscle fibres in the m. lateralis of the European eel Anguilla anguilla (L.) (Pisces, Teleostei). J Anat 125: 447–460
Yablonka-Reuveni Z (1988) Discrimination of myogenic and nonmyogenic cells from embryonic skeletal muscle by 90° light scattering. Cytometry 9: 121–125
Yablonka-Reuveni Z, Quinn LS, Nameroff M (1987) Isolation and clonal analysis of satellite cells from chicken pectoralis muscle. Dev Biol 119: 252–259
Yaffe D (1968) Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc Natl Acad Sci USA 61: 477–483
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koumans, J.T.M., Akster, H.A., Dulos, G.J. et al. Myosatellite cells of Cyprinus carpio (Teleostei) in vitro: isolation, recognition and differentiation. Cell Tissue Res 261, 173–181 (1990). https://doi.org/10.1007/BF00329450
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00329450