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Composition and morphology of Candida utilis grown in continuous culture with decreasing concentrations of phosphate

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Abstract

Candida utilis was grown in continuous culture with decreasing concentrations of phosphate. At a constant dllution rate three successlve growth patterns were observed: carbon-limited growth, carbon and phosphate dual-limited growth, and phosphatelimited growth. Phosphate deficlency as well as phosphate limitation produced significant modifications in cell composition and morphology, including cell size. At the lowest phosphate concentration (6 mg/P/I), the cell size was the largest and the relative contents of phosphorus and RNA in the cells was very low, with 96% of the total phosphorus being located in the RNA fraction.

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M.E. Lucca and M.E. Romero are with the Cátedra de Microbiología Industrial, Fac. Bioq. y Farmacia (U.N.T.) C.C. 90, suc. 2 (4.000) S.M. Tucumán, Argentina, J.C. Díaz Ricci, O.A. Garro and D.A.S. Callieri are with PROIMI, Avda Belgrano y Pje Caseros (4000) S.M. Tucumán, Argentina.

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Lucca, M.E., Romero, M.E., Díaz Ricci, J.C. et al. Composition and morphology of Candida utilis grown in continuous culture with decreasing concentrations of phosphate. World Journal of Microbiology and Biotechnology 7, 359–364 (1991). https://doi.org/10.1007/BF00329403

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  • DOI: https://doi.org/10.1007/BF00329403

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