Abstract
The activity of Bordetella pertussis extracytoplasmic adenylate cyclase (AC) decreased during decelerating growth phase in a Stainer-Scholte medium. Neither proteolytic activity nor virulence variation (phase variation; antigenic modulation) appears to be responsible for the observed activity fall. The addition of methyl-β-cyclo-dextrin enhances AC activity and prevents the inhibition of AC activity by fatty acids. Cyclodextrin could entrap inhibitors increasing in this way the AC activity. These results show that the inclusion of cyclodextrin in the culture medium increases the AC activity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Armstrong, S. & Parker, C. 1986 Surface proteins of Bordetella pertussis: comparison of virulent and avirulent strains and effects of phenotypic modulation. Infection and Immunity 54, 308–314.
Bellalou, J. & Relyveld, E. 1984 Studies on culture conditions of Bordetella pertussis and relationship to immunogenitity of vaccines. Annales d'Institute Pasteur/Microbiologie 1353, 101–110.
Black, W., Munoz, J., Peacock, M., Schad, P., Cowell, J., Burchall, J., Lim, M., Kent, A., Steinman, L. & Falkow, S. 1988 ADP-ribosiltransferase activity of pertussis toxin and immunomodulation of Bordetella pertussis. Science 240, 656–659.
Brezin, C., Guiso, N., Ladant, D., Djavadi-Ohaniance, L., Megret, F., Onyeocha, I. & Alonso, J. 1987 Protective effect of anti-Bordetella pertussis adenylate cyclase antibodies against lethal respiratory infection of the mouse. FEMS Microbiology Letters 43, 75–80.
Brownlie, R., Coote, J., Parton, R., Schultz, J., Rogel, A. & Hanski, E. (1988) Cloning of the adenylate cyclase gentic determinant of B. pertussis and its expression in Escherichia coli. Microbial Pathogenesis, 335–344.
Flawia, M. & Torres, H. 1972 Adenylate cyclase in Neurospora crassa I. Journal of Biological Chemistry 243, 68–73.
Goldman, S., Hanski, E. & Fish, F. 1984 Spontaneous phase variation in Bordetella pertussis is multistep non-random process. EMBO Journal 3, 1353–1356.
Guiso, N., Rocancourt, M., Szatanik, M. & Alonso, J. 1989 Bordetella adenylate cyclase is a virulence associated factor and immunoprotective antigen. Microbial Pathogenesis 7, 373–380.
Hewlett, E., Gordon, V., McCaffery, J., Sutherland, W. & Gray, M. 1989 Adenylate cyclase toxin from Bordetella pertussis. Identification and purification of holotoxin molecule. Journal of Biological Chemistry 264, 1937–1938.
Imaizumi, A., Suzuki, Y., Ono, S. & Sato, Y. 1983 Heptakis (2,6-0-dimethyl) betacyclodextrin: a novel growth stimulant for Bordetella pertussis phase I. Journal of Clinical Microbiology 17, 781–786.
Kessin, R. & Franke, J. 1986 Secreted adenylate cyclase of Bordetella pertussis: calmodulin requirements and partial purification of two forms. Journal of Bacteriology 166, 290–296.
Munoz, J., Arai, H. & Cole, R. 1981 Mouse-protecting and Histamine-sensitizing activities of pertussigen and fimbrial haemagglutinin from Bordetella pertussis. Infection and Immunity 32, 243–250.
Robinson, A., Irons, L. & Ashworth, L. 1985 Pertussis vaccine: present status and future prospects. Vaccine 3, 11–22.
Sato, Y., Kimura, M. & Fukumi, H. 1984 Development of a pertussis components vaccine in Japan. Lancet 1, 122–126.
Schnaitman, C. 1971 Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. Journal of Bacteriology 108, 545–552.
Schneider, D. & Parker, C. 1982 Effect of pyridines on phenotypic properties of Bordetella pertussis. Infection and Immunity 38, 548–553.
Stainer, D. & Scholte, M. 1971 A simple chemically defined medium for the production of phase I Bordetella pertussis. Journal of General Microbiology 63, 211–220.
Starkcoff, A. & Szabo, L. 1986 The fatty acids content of the Bordetella pertussis endotoxin. Journal of General Microbiology 132, 97–102.
Suzuki, Y., Imaizumi, A., Ginnaga, A., Satto, H. & Sato, Y. 1985 Effect of Heptakis (2,6-0-dimethyl) beta-cyclodextrin on cell growth and the production of pertussis toxin and filamentous haemagglutinin in Bordetella pertussis. Development in Biological Standardization 61, 89–92.
Urisu, A., Cowell, J. & Manclark, C. 1986 Filamentous haemagglutinin has a major role in mediating adherence of Bordetella pertussis to human WiDr cells. Infection and Immunity 52, 695–701.
Weiss, A. & Falkow, S. 1984 Genetic analysis of phase change in Bordetella pertussis 43, 263–269.
Weiss, A., Hewlett, E., Myers, G. & Falkow, S. 1985 Pertussis toxin and extracytoplasmic adenylate cyclase as virulence factor of Bordetella pertussis. Journal of Infectious Diseases 150, 219–222.
Additional information
D.F. Hozbor and O.M. Yantorno are with the Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, (1900) La Plata, Argentina. A. Samo is with the Comisión de Investigaciones Cientificas de la Provincia de Buenos Aires.
Rights and permissions
About this article
Cite this article
Hozbor, D.F., Samo, A. & Yantorno, O.M. Effect of methyl-cyclodextrin on adenylate cyclase activity of Bordetella pertussis . World Journal of Microbiology and Biotechnology 7, 309–315 (1991). https://doi.org/10.1007/BF00329396
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00329396