Summary
Rats infected with Trypanosoma lewisi were studied for more than 500 days to measure the duration of the immune state. Sixty rats arranged in groups of ten were each inoculated with 100 cells of T. lewisi on Day 1. Subsequently, the first group was re-inoculated with the same number of trypanosomes on Day 56. On Day 141 the first group was again challenged and the second group was challenged for the first time. On Day 234 the first group was reexposed for the third time; the second group for the second, and the third group of rats was reexposed for the first time. This pattern was continued at Days 324, 414 and 505 when the 1st group was reexposed for the first time. Additional rats were inoculated on each challenge date for control of viability of the trypanosomes.
Techniques for detection of parasites in the host's body were: (a) wet films of tail blood; (b) permanent blood smears; and (c) subinoculation of host blood into susceptible animals (xenodiagnosis).
The immune state was steady in all groups during the periods of observation. No active trypanosomes were detected at any period of observation. However, inactive or dead trypanosomes were found in the peripheral blood of rats reinoculated on Day 414 and 505. Control animals showed active parasitemias during each reinoculation period.
Similar content being viewed by others
References
Corradetti, A., Verolini, F.: Comportamento di Trypanosoma lewisi in ratit albini reinfettai con ceppo omologo prima o dopo la splecectomia. Parassitologia 1, 1–10 (1959).
Corradetti, A., Verolini, F.: Decorso della reinfezione da Plasmodium berghei in ratti albino precendentemente infettati, guariti e splenectomizzati. Parassitologia 2, 99–103 (1960).
Coventry, F. A.: The reaction product which inhibits reproduction of the trypanosomes in infections with Trypanosoma lewisi, with special references to its changes in titer throughout the course of the infection. Amer. J. Hyg. 5, 127–144 (1925).
Coventry, F. A.: The trypanocidal actions of specific antiserums on Trypanosoma lewisi in vivo. Amer. J. Hyg. 12, 366–380 (1930).
D'Alesandro, P. A.: Immunological and biochemical studies of ablastin, the reproduction inhibiting antibody of Trypanosoma lewisi. Ann. N. Y. Acad. Sci. 129, 834–852 (1966).
Greenblatt, C. L., Lincicome, D. R.: Identity of trypanosome growth factors in serum. II. Active globulin components. Exp. Parasit. 19, 139–150 (1966).
Hornby, H. E.: Animal trypanosomes in eastern Africa 1949. London: H. M. S. O. 1952.
Lincicome, D. R., Hill, G. C.: Oxygen uptake by Trypanosoma lewisi complex cells I. “L” isolate. Comp. Biochem. Physiol. 14, 425–435 (1965).
Lincicome, D. R., Watkins, R. C.: Method for preparing pure cell suspensions of Trypanosoma lewisi. Amer. Inst. Biol. Sci. Bull. 13, 53–54 (1963).
Novy, F. G., MacNeal, W. J.: On the cultivation of Trypanosoma brucei. J. infect. Dis. 1, 1–30 (1904).
Packchanian, A.: Experimental Trypanosoma brucei infection and immunity in various species of Peromyscus (American deer mice). Amer. J. Hyg. 20, 135–147 (1934).
Soltys, M. A., Woo, P.: Multiplication of Trypanosoma brucei and Trypanosoma congolense in vertebrate hosts. Trans. roy. Soc. trop. Med. Hyg. 63 (4), 490–494 (1969).
Soltys, M. A., Woo, P.: Further studies on tissue forms of Trypanosoma brucei in a vertebrate host. Trans. roy. Soc. trop. Med. Hyg. 64, 992–694 (1970).
Soltys, M. A., Woo, P., Gillick, A. C.: A preliminary note on the separation and infectivity of tissue forms of Trypanosoma brucei. Trans. roy. Soc. trop. Med. Hyg. 64, 495–496 (1969).
Soulsby, E. J. L.: Antigen-antibody reactions in helminth infections. Advanc. Immunol. 2, 265–303 (1962).
Taliaferro, W. H.: A reaction product in infection with Trypanosoma lewisi which inhibits the reproduction of the trypanosomes. J. of exp. Med. 39, 171–190 (1924).
Taliaferro, W. H.: The immunology of parasitic infections. New York: Century Company N. Y. 1929.
Taliaferro, W. H.: Trypanocidal and reproduction inhibiting antibodies to Trypanosoma lewisi in rats and rabbits. Amer. J. Hyg. 16, 32–84 (1932).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, C.M., Lincicome, D.R. Trypanosoma lewisi: Duration of immune state in rats. Z. Parasitenk. 38, 344–350 (1972). https://doi.org/10.1007/BF00329281
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00329281