Summary
Isoelectric focusing (IEF) is widely used for the determination of serum protein polymorphisms in forensic investigations. Because of non-genetic heterogeneity of some proteins and of protein-ligand dissociation observed after IEF standardization of procedures is necessary. In this article the authors point out the main difficulties encountered in studying the transferrin subtype polymorphism and describe a new and sensitive method. Two new TfC subtypes were observed: TfC4 in North American, Bolivian and Brazilian Indians, TfC5 in Black Americans, both with polymorphic allele frequencies. In a Pyrenean population the allele frequencies for TfC1, TfC2 and TfC3 were quite similar to those observed in a German population sample.
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Constans, J., Kühnl, P., Viau, M. et al. A new procedure for the determination of transferrinC (TfC) subtypes by isoelectric focusing. Hum. Genet. 55, 111–114 (1980). https://doi.org/10.1007/BF00329135
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DOI: https://doi.org/10.1007/BF00329135