Abstract
A simple experiment was carried out to examlne the effect of varlous physicochemical conditions on toxigenicity and toxin genes of Vibrio cholerae 01 lsolated from an artificial aquatic environment. All isolated strains, tested by tissue culture assay, DNA-DNA hybridization and ELISA, were cytotoxic to Vero cells, did not lose their toxin genes and continued to produce cholera toxin. These results are consistent with the hypothesis that V. cholerae can survive in the environment without losing potential pathogenicity.
References
Colwell, R.R., Singleton, F.L., Huq, A., Xu, H.S. & Roberts, N. 1985 Ecology of Vibrio cholerae, Vibrio parahaemolyticus and related vibrios in the natural environment. In Bacterial Diarrheal Diseases, eds Takeda, Y. & Miwatani, T. pp. 273–295. Tokyo: KTK Scientific Publishers.
Feachem, R.G., Miller, C.J. & Drasar, B.S. 1981 Environmental aspects of cholera epidemiology II. Occurrence and survival of Vibrio cholerae in the environment. Tropical Diseases Bulletin 78, 865–880.
Grunstein, M. & Hogness, D.S. 1975 Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences of the USA 72, 3961–3965.
Islam, M.S. 1990a Effects of various biophysicochemical conditions on toxigenicity of Vibrio cholerae 01 during survival with a green alga, Rhizoclonium fontanum in artificial aquatic environment. Canadian Journal of Microbiology 36, 464–468.
Islam, M.S. 1990b Increased toxin production by Vibrio cholerae 01 during survival with a green alga, Rhizoclonium fontanum in artificial aquatic environment. Microbiology and Immunology 34, 557–563.
Islam, M.S., Drasar, B.S. & Bradley, D.J. 1989 Attachment of toxigenic Vibrio cholerae 01 to various freshwater plants and survival with a filamentous green alga, Rhizoclonium fontanum. Journal of Tropical Medicine and Hygiene 92, 396–401.
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Islam, S. Toxigenicity and toxin genes of Vibrio cholerae 01 isolated from an artificial aquatic environment. World Journal of Microbiology and Biotechnology 7, 269–271 (1991). https://doi.org/10.1007/BF00329000
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DOI: https://doi.org/10.1007/BF00329000