Skip to main content
SpringerLink
Log in

DNA of Chinese hamster V79 cells newly synthesized after ultraviolet irradiation contains alkali-labile sites

  • Short Communication
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Pulse-labeled daughter DNA of UV-irradiated Chinese hamster V79 cells was denatured in alkaline or neutral conditions and analysed by sucrose gradient centrifugation. A comparison of the sedimentation profiles of DNA treated in alkaline or neutral conditions has shown that in UV-irradiated cells some alkali-labile sites are produced during replication of damaged templates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Buhl SN, Setlow RB, Regan JD (1973) Recovery of the ability to synthesize DNA in segments of normal size at long times after ultraviolet irradiation of human cells. Biophys J 13:1265–1275

    Google Scholar 

  • Burgi E, Hershey AD (1963) Sedimentation rate as a measure of molecular weight of DNA. Biophys J 3:309–321

    Google Scholar 

  • Cupido M (1983) Bypass of pyrimidine dimers in DNA of bacteriophage T4 via induction of primer RNA. Mutat Res 109:1–11

    Google Scholar 

  • Elkind MM, Kamper C (1970) Two forms of repair of DNA in mammalian cells following irradiation. Biophys J 10237–245

    Google Scholar 

  • Lehmann AR, Kirk-Bell S (1972) Post-replication repair of DNA in ultraviolet-irradiated mammalian cells. No gaps in DNA synthesized late after ultraviolet irradiation. Eur J Biochem 31:438–445

    Google Scholar 

  • Meyn RE, Humphrey RM (1971) Deoxyribonucleic acid synthesis in ultraviolet-light-irradiated Chinese hamster cells. Biophys J 11:295–301

    Google Scholar 

  • Setlow RB, Regan JD, German J, Carrier WL (1969) Evidence that Xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA, Proc Natl Acad Sci USA 64:1035–1041

    Google Scholar 

  • Slezáriková V, Sedliaková M (1982) The presence of alkali-labile sites in DNA daughter chains of UV-irradiated Escherichia coli. FEBS Lett 148:251–254

    Google Scholar 

  • Studier FW (1965) Sedimentation studies of the size and shape of DNA. J Mol Biol 11:373–390

    Google Scholar 

  • Veatch W, Okada S (1969) Radiation-induced breaks of DNA in cultured mammalian cells. Biophys J 9:330–346

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, CS-812 32, Bratislava, Czechoslovakia

    Miroslav Piršel & Milena Sedliaková

Authors
  1. Miroslav Piršel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Milena Sedliaková
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Böhme

Rights and permissions

Reprints and permissions

About this article

Cite this article

Piršel, M., Sedliaková, M. DNA of Chinese hamster V79 cells newly synthesized after ultraviolet irradiation contains alkali-labile sites. Molec Gen Genet 198, 183–184 (1984). https://doi.org/10.1007/BF00328722

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00328722

Keywords

Search

Navigation