Summary
Adult human testicular tissue contains up to six previously undescribed lactate dehydrogenase (LDH) isoenzymes in addition to the five LDH isoenzymes normally found and the sixth found in spermatogenic cells and spermatozoa, LDH-X. Additional LDH isoenzymes were also found in spermatozoa but not in seminal fluid or in serum. After electrophoresis one additional LDH isoenzyme of testicular tissue was localized between LDH-1 and LDH-2, two between LDH-2 and LDH-3, two between LDH-3 and LDH-4, and two between LDH-4 and LDH-5. These localizations indicate that the additional LDH isoenzymes are tetramers combining the A and B subunits of the five normal LDH isoenzymes and the C subunit of LDH-X. The additional LDH isoenzymes may be important in the metabolism of spermatogenic germ cells and spermatozoa.
Similar content being viewed by others
References
Blanco A (1980) On the functional significance of LDHX. Johns Hopkins Med J 146:231–235
Blanco A, Zinkham WH (1963) Lactate dehydrogenases in human testes. Science 139:601–602
Blanco A, Zinkham WH, Kupchyk L (1964) Genetic control and ontogeny of lactate dehydrogenase in pigeon testes. J Exp Zool 156:137–152
Eyben FE von, Skude G, Trope C, Wennerberg J, Mikulowski P (1982) Lactate dehydrogenase isoenzyme 1 (LDH-1) in a thymic mice with xenografts of a human testicular germ cell tumor. Mol Gen Genet 186:427–431
Eyben FE von, Skude G, Fosså SD, Klepp O, Børmer O (1983) Serum lactate dehydrogenase (S-LDH) and S-LDH isoenzymes in patients with testicular germ cell tumors. Mol Gen Genet 189:326–333
Goldberg E (1963) Lactic and malic dehydrogenases in human spermatozoa. Science 139:602–603
Lundh B (1967) A macromolecular serum lactate dehydrogenase activity in a case of leukemia. Clin Chim Acta 36:305–309
Meistrich ML, Trostle PK, Frapart M, Erickson RP (1977) Biosynthesis and localization of lactate dehydrogenase X in pachytene spermatocytes and spermatids of mouse testes. Dev Biol 60:428–441
Niklasson O, Skude G, Johansson R, Stormby N (1981) Screening of endometrial carcinoma by lactate dehydrogenase isoenzyme analysis of uterine fluid. Acta Obstet Gynecol Scand 60:1–8
The Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinial Physiology (1974) Recommended methods for the determination of four enzymes in blood. Scand J Clin Lab Invest 33:291–306
Zinkham WH, Blanco A, Kupchyk L (1963) Lactate dehydrogenase in testis: Dissociation and recombination of subunits. Science 142:1303–1304
Zinkham WH, blanco A, Clowry LJ, Jr (1964) An unusual isozyme of lactate dehydrogenase in mature testes: localization, ontogeny, and kinetic properties. Ann NY Acad Science 121:571–588
Author information
Authors and Affiliations
Additional information
Communicated by K. Illmense
Rights and permissions
About this article
Cite this article
Skude, G., von Eyben, F.E. & Kristiansen, P. Additional lactate dehydrogenase (LDH) isoenzymes in normal testis and spermatozoa of adult man. Molec Gen Genet 198, 172–174 (1984). https://doi.org/10.1007/BF00328718
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00328718