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Transposon Tn5 mutagenesis of genes for the photosynthetic apparatus in Rhodopseudomonas capsulata

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Summary

Three plasmids containing the transposon Tn5, i.e. pSUP201::Tn5, pACYC184::Tn5 and pJB4JI were transferred from Escherichia coli to Rhodopseudomonas capsulata in order to mutagenize the genome. Mutants defective in bacteriochlorophyll and carotenoid synthesis and mutants unable to form the photochemical reaction center or one of the light-harvesting complexes were isolated. Of special interest were mutants that could not form the light-harvesting complex B800-850. Two of these mutants synthesized only two of the three polypeptides of this complex whereas the corresponding near infrared absorbance bands were not observed. Complementation analysis with the Rprime plasmid pRPS404, which contains a 50 kb region of the genome of R. capsulata carrying most genes responsible for expression of photosynthetic apparatus, revealed that some genes of the B800-850 light-harvesting complex lie outside this photosynthetic gene cluster.

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Abbreviations

Bchl:

Bacteriochlorophyll

Cm:

chloramphenicol

Km:

kanamycin

Tc:

tetracycline

Ap:

ampicillin

Gm:

gentamicin

Spc:

spectinomycin

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Authors and Affiliations

  1. Institur für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Schänzlestraße 1, D-7800, Freiburg, Federal Republic of Germany

    Norbert Kaufmann, Hendrik Hüdig & Gerhart Drews

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  1. Norbert Kaufmann
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  2. Hendrik Hüdig
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  3. Gerhart Drews
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Communicated by A. Böck

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Kaufmann, N., Hüdig, H. & Drews, G. Transposon Tn5 mutagenesis of genes for the photosynthetic apparatus in Rhodopseudomonas capsulata . Molec Gen Genet 198, 153–158 (1984). https://doi.org/10.1007/BF00328715

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  • DOI: https://doi.org/10.1007/BF00328715

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