Summary
Salmonella phage mutants P22 cir4-1, P22 cir5-1 and P22 cir6-1 at 37°C form more or less clear plaques. The mutants complement each other and clear mutations of the immC region (Prell et al. 1983). The mutants exhibit a strongly reduced frequency of lysogenisation, but form stable prophages. The low frequency of lysogenisation of P22 cir5-1 and of P22 cir6-1 is suppressed by an additional ant - mutation. Similarly, about 50% of turbid plaque revertants of both mutants carry ant - suppressor mutations. This suggests interference by the cir5-1 and cir6-1 mutations with the expression of gene ant. In contrast, the cir4-1 mutation seems not to interfere with ant expression, the latent periods of P22 cir4-1 and P22 cir5-1 are reduced and extended, respectively. The geneticly related Salmonella phage L carries a gene able to complement P22 cir4-1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bezdek M, Amati P (1968) Evidence for two immunity regulator systems in temperate bacteriophage P22 and L. Virology 36:701–703
Bezdek M, Soska J, Amati P (1970) Properties of P22 and a related Salmonella typhimurium phage. III. Studies on clear-plaque mutants of phage L. Virology 40:505–513
Botstein D, Lew KK, Jarvik V, Swanson CA Jr (1975) Role of antirepressor in the bipartite control of repression and immunity by bacteriophage P22. J Mol Biol 91:439–462
Gough M (1968) Second locus of bacteriophage P22 necessary for the maintenance of lysogeny. J Virol 2:992–998
Harvey AM, Hava P, Oppenheim AB, Prell HH, Soska J (1981) Repression of ant synthesis early in the lytic cycle of phage P22. Mol Gen Genet 181:74–81
Harvey AM, Heil J, Prell HH (1979) Repressor synthesis in regulatory mutants of bacteriophage P22. Mol Gen Genet 167:337–339
Hilliker S (1981) Characterization of the Salmonella phage L early genes using immI hybrid phages. Virology 114:161–174
Kahmann R, Prell HH (1971) Complementation between P22 amber mutants and phage L. Mol Gen Genet 113:363–366
Levine M (1957) Mutations in the temperate phage P22 and lysogeny in Salmonella. Virology 3:22–41
Levine M, Truesdell S, Ramakrishnan T, Bronson MJ (1975) An antirepressor locus and its controlling elements. J Mol Biol 91:421–438
Prell HH (1970a) Px, a hybrid between the serological unrelated and heteroimmune Salmonella bacteriophages P22 and Py. I. Some properties of Py and Px, genetic evidence for the hybrid nature of Px and phenotypic mixing between P22, Py and Px. Mol Gen Genet 108:167–183
Prell HH (1970b) Px, a hybrid between the serological unrelated and heteroimmune Salmonella bacteriophages P22 and Py. II. Contribution of P22 genetic material to different Px variants. Mol Gen Genet 108:184–202
Prell HH (1973) Regulation of gene expression in Salmonella phage P22. I. Genetic experiments involving P22 and Px1. Mol Gen Genet 127:327–339
Prell HH (1975) Regulation of gene expression in Salmonella phage P22. II. Regulation of expression of late functions Mol Gen Genet 136:351–360
Prell HH (1977) The role of ant-product of Salmonella phage P22 in the process of transactivation of prophage Px1. Mol Gen Genet 156:61–69
Prell HH (1978) The activity of ant product of the Salmonella phage P22 against the closely related but heteroimmune phage L. Mol Gen Genet 161:197–204
Prell HH, Auer JM, Soska J (1983) Clear plaque mutation sites linked to the immI region of the Salmonella phage P22. Genet Res 42:267–283
Prell HH, Auer JM, Soska J (1984) The immI region of Salmonella phage P22. 2. The influence of the cir-mutations on the regulation of antirepressor synthesis. Mol Gen Genet 198:110–115
Susskind MM, Botstein D (1978) Molecular genetics of bacteriophage P22. Microbiology Reviews 42:385–413
Susskind MM, Youderian P (1982) Transcription in vitro of the bacteriophage P22 antirepressor gene. J Mol Biol 154:427–447
Youderian P, Chadwick SJ, Susskind MM (1982) Autogenous regulation by the bacteriophage P22 arc gene product. J Mol Biol 154:449–464
Whitfield HJ Jr, Martin RG, Ames BN (1966) Classification of aminotransferase (C gene) mutants in the histidine operon. J Mol Biol 21:335–355
Zinder ND (1958) Lysogenization and superinfection immunity in Salmonella. Virology 5:291–336
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Prell, H.H., Auer, J., Soška, J. et al. The immI region of Salmonella phage P22. Molec Gen Genet 198, 105–109 (1984). https://doi.org/10.1007/BF00328708
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00328708