Summary
A 37 kb fragment of DNA from an F-prime factor, F100-12, which showed a gene dosage effect on b-type cytochromes, was cloned with a cosmid vector, pHC79. Gel filtration of cytochromes and product analysis of the hybrid plasmids indicated that this fragment contained cybB, the structural gene for cytochrome b561. A chromosomal DNA fragment carrying the cybB gene was cloned by the plaque hybridization technique with Charon 4A as a vector. The gene was subcloned into pBR322 and was located in a 1.3 kb DNA fragment. It was concluded that the cybB gene is located on the chromosome of Escherichia coli K12.
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Abbreviations
- SDS:
-
sodium dodecyl sulfate
- PAGE:
-
polyacrylamide gel electrophoresis
- HPLC:
-
high performance liquid chromatography
- NADH:
-
reduced form of nicotinamide adenine dinucleotide
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Communicated by M. Takanami
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Murakami, H., Kita, K. & Anraku, Y. Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12. Molec Gen Genet 198, 1–6 (1984). https://doi.org/10.1007/BF00328692
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DOI: https://doi.org/10.1007/BF00328692