Summary
A tetraploid cell population was produced in the primary root meristem of Pisum sativum by one-half hour treatments with various concentrations of colchicine. The tetraploid population so produced was found to be reasonably synchronous in its passage through successive mitotic cycles with the degree of synchrony being more or less proportional to the concentration of colchicine used. The average time between mitoses appears to be of the order of 12 hours which agrees well with previous estimates. Treatments of roots containing tetraploid populations with 2.52 × 10−5 M. actidione for 15 minutes were used to demonstrate the possibility of using the system for studies on the differential susceptibility of cells at different stages of the mitotic cycle.
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References
Campbell, A.: Synchronization of cell division. Bact. Rev. 21, 263–272 (1957).
Caspersson, T.: Über die Rolle der Desoxyribosenukleinsäure bei der Zellteilung. Chromosoma (Berl.) 1, 147–156 (1939).
Evans, H. J., G. J. Neary and S. M. Tonkinson: The use of colchicine as an indicator of mitotic rate in broad bean root meristems. J. Genet. 55, 487–502 (1957).
Hadder, J. C., and G. B. Wilson: Cytological assay of c-mitosis and prophase poison reactions. Chromosoma (Berl.) 9, 91–104 (1958).
Heilbrunn, L. V.: The dynamics of living protoplasm. New York: Academic Press, Inc. 1956.
Hunter-Szybalska, E. M., W. Szybalska and E. D. Delamater: Temperature synchronization of nuclear and cellular division in Bacillus megaterium. J. Bact. 71, 17–24 (1956).
James, T. W.: Synchronization of cell division in Amoebae. Ann. N.Y. Acad. Sci. 78, 501–514 (1959).
Levan, A.: The effect of colchicine on the root mitosis in Allium. Hereditas (Lund) 24, 471–486 (1938).
—: Colchicine induced c-mitosis in two mouse ascites tumors. Hereditas (Lund) 40, 1–64 (1954).
Mazia, D., and K. Dan: The isolation and biochemical characterization of the mitotic apparatus of dividing cells. Proc. Nat. Acad. Sci. (Wash.) 38, 826–838 (1952).
Moses, M. J., and J. H. Taylor: Desoxypentose nucleic acid synthesis during microgametogenesis in Tradescantia. Exp. Cell Res. 9, 474–488 (1955).
Scherbaum, O., and E. Zeuthen: Induction of synchronous cell division in mass cultures of Tetrahymena pyriformis. Exp. Cell Res. 6, 221–227 (1954).
Sparrow, A. H., and R. C. Sparrow: Treatment of Trillium erectum prior to and during mass production of permanent smear preparations. Stain Technol. 24, 47–55 (1949).
Taylor, J. H.: Autoradiographic detection of incorporation of P32 into chromosomes during meiosis and mitosis. Exp. Cell Res. 4, 169–179 (1953).
Wilson, G. B., and J. H. Morrison: The mitotic cycle and the ontogeny of neoplastic growth. Cytologia 24, 43–49 (1959).
—, A. H. Sparrow and V. Pond: Sub-chromatid rearrangements in Trillium erectum. I. Origin and nature of configurations induced by ionizing radiation. Amer. J. Bot. 46, 309–316 (1959).
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This work was carried out under contract number RG-4835 of the National Institutes of Health, United States Public Health Service, and an Institutional Grant from the American Cancer Society.
Contribution number 59-26 of the Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan.
Predoctoral Fellow (CF-9871) of the National Cancer Institute, United States Public Health Service
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Van't Hof, J., Wilson, G.B. & Colon, A. Studies on the control of mitotic activity. Chromosoma 11, 313–321 (1960). https://doi.org/10.1007/BF00328658
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DOI: https://doi.org/10.1007/BF00328658