Abstract
A family of dispersed repeats longer than 7 kilobase pairs (kbp) has been identified in the very large genome of Lilium henryi, and two subregions cloned. Initially a rapidly reannealing probe (C0t<1 M s) was prepared by hydroxyapatite chromatography. Half the copies of all sequences repeated 15000 times per genome are expected to reanneal by this C0t value. The probe hydridized to abundant fragments of 2, 5, and 7 kbp released from genomic DNA by Bam HI digestion. Twelve 2-kb fragments and ten 5-kb sequences were cloned into pBR322. Restriction mapping of the two sets of clones showed individual members to be quite similar. Length variation was no more than 200 base pairs (bp) between repeats, and consensus sites were present on 80%–90% of occasions. In situ hybridization using representative 2-kbp and 5-kbp clones showed each sequence to be dispersed throughout all chromosomal regions. Studies on the genomic organization suggested that the 2-kbp and 5-kbp sequences are usually adjacent, and that occasional absence of the internal Bam HI site results in the release of the 7-kbP fragment. There are at least 13000 copies of the full repeat per L. henryi genome, thus accounting for approximately 0.3% of the total of 32 million kbp.
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References
Appels R, Bouchard RA, Stern H (1982) cDNA clones from meiotic-specific poly(A)+ RNA in Lilium: Homology with sequences in wheat, rye, and maize. Chromosoma 85:591–602
Bennett MB, Smith JB (1976) Nuclear DNA amounts in angiosperms. Phil Trans R Soc London Ser B 274:227–274
Bouchard RA (1982) Moderately repetitive DNA in evolution. Int Rev Cytol 76:113–193
Bouchard RA, Stern H (1980) DNA synthesized at pachytene in Lilium: A non-divergent subclass of moderately repetitive sequences. Chromosoma 81:349–363
Clewell DB, Helinski DR (1972) Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol 110:1135–1146
Evans IJ, James AM, Barnes SR (1983) Organization and evolution of repeated DNA sequences in closely related plant genomes. J Mol Biol 170:803–826
Flavell RB, Smith DB (1977) Nucleotide sequence organisation in the rye genome. Biochim Biophys Acta 474:82–97
Flavell RB, O'Dell M, Hutchinson J (1981) Nucleotide sequence organization in plant chromosomes and evidence for sequence translocation during evolution. Cold Spring Harbor Symp Quant Biol 45:501–508
Freeling M (1984) Plant transposable elements and insertion sequences. Ann Rev Plant Physiol 35:277–298
Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Goldberg RB (1978) DNA sequence organization in the soybean plant. Biochem Genet 16:45–68
Graham DE (1978) The isolation of high molecular weight DNA from whole organisms of large tissue mass. Anal Biochem 85:609–613
Gupta M, Bertram I, Shepherd NS, Saedler H (1983) Cin 1, a family of dispersed repetitive elements in Zea mays. Mol Gen Genet 192:373–377
Gupta M, Shepherd NS, Bertram I, Saedler H (1984) Repetitive sequences and their organization on genomic clones of Zea mays. EMBO 3:133–139
Hake S, Walbot V (1980) The genome of Zea mays, its organization and homology to related grasses. Chromosoma 79:251–270
Hotta Y, Stern H (1975) Zygotene and pachytene labeled sequences in the meiotic organization of chromosomes. In: Peacock WJ, Brock RD (eds) The eukaryote chromosome. ANU Press, Canberra, pp 283–300
Kiper M, Herzfeld F (1978) DNA sequence organization in the genome of Petroselinum sativum (Umbelliferae). Chromosoma 65:335–351
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 98–104
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Rimpau J, Smith D, Flavell R (1978) Sequence organisation analysis of the wheat and rye genomes by interspecies DNA/DNA hybridization. J Mol Biol 123:327–359
Smith DB, Flavell RB (1977) Nucleotide sequence organisation in the rye genome. Biochim Biophys Acta 474:82–97
Smyth DR, Stern H (1973) Repeated DNA synthesized during pachytene in Lilium henryi. Nature New Biol 245:94–96
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
von Kalm L, Smyth DR (1984) Ribosomal RNA genes and the substructure of nucleolar organizing regions in Lilium. Can J Genet Cytol 26:158–166
Walbot R, Goldberg R (1979) Plant genome organization and its relationship to classical plant genetics. In: Hall TC, Davies JW (eds) Nucleic acids in plants. CRC Press, Boca Raton, pp 3–40
Zimmerman JL, Goldberg RB (1977) DNA sequence organization in the genome of Nicotiana tabacum. Chromosoma 59:227–252
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Sentry, J.W., Smyth, D.R. A family of repeated sequences dispersed through the genome of Lilium henryi . Chromosoma 92, 149–155 (1985). https://doi.org/10.1007/BF00328467
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DOI: https://doi.org/10.1007/BF00328467