Abstract
Examination of early embryos of Drosophila virilis by light and electron microscopy has shown that the A-T rich satellite DNA sequences have a non-random distribution within the nuclei. As observed by 33258 Hoechst staining and fluorescent microscopy, these sequences are consistently found to be located on the sides of the nuclei nearest to the vitelline membrane. This arrangement of the A-T rich sequences has been observed from the syncytial balstoderm stage into the gastrula stage where, in each nucleus, the satellite DNA sequences remain at a point nearest the topological outside of the organism.
Similar content being viewed by others
References
Bryant, P.J., Schneiderman, H.: Cell lineage, growth, and determination in the imaginal leg discs of Drosophila melanogaster. Develop. Biol. 20, 262–290 (1969)
Fullilove, S.L., Jacobson, A.G.: Nuclear elongation and cytokinesis in Drosophila montana. Develop. Biol. 26, 560–577 (1971)
Fullilove, S.L., Jacobson, A.G.: Embryonic development: Descriptive. In: The genetics and biology of Drosophila (M. Ashburner and T.R.F. Wright, eds.), Vol. 2c, p. 106–229. New York: Academic Press 1978
Gall, J.G., Atherton, D.O.: Satellite DNA sequences in Drosophila virilis. J. molec. Biol. 85, 633–634 (1974)
Gall, J.G., Cohen, E., Polan, M.L.: Repetitive DNA sequences in Drosophila. Chromosoma (Berl.) 33, 319–344 (1971)
Hennig, W., Hennig, I., Stein, H.: Repeated sequences in the DNA of Drosophila and their localization in giant chromosomes. Chromosoma (Berl.) 32, 31–63 (1970)
Holmquist, G.: Hoechst 33258 fluorescent staining of Drosophila chromosomes. Chromosoma (Berl.) 49, 333–356 (1975)
Huettner, A.F.: Continuity of the centrioles in Drosophila melanogaster. Z. Zellforsch. 19, 119–134 (1933)
Latt, S.A.: Microfluormetic detection of DNA replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
Mahowald, A.P.: Ultrastructural differentiations during formation of the blastoderm in Drosophila melanogaster embryo. Develop. Biol. 8, 186–204 (1963)
Mayfield, J.E., Ellison, J.R.: The organization of the interphase chromatin in Drosophilidae, the self adhesion of chromatin containing the same DNA sequences. Chromosoma (Berl.) 52, 37–48 (1975)
Peacock, W.T., Brutlag, D., Goldring, E., Appels, R., Hinton, C.W., Lindsley, D.L.: The organization of highly repeated DNA sequences in Drosophila melanogaster chromosomes. Cold Spr. Harb. Symp. quant. Biol. 38, 405–416 (1973)
Rae, P.M.M.: Chromosomal distribution of rapidly reannealing DNA in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 67, 1018–1025 (1970)
Sonnenblick, B.P.: The early embeyology of Drosophila melanogaster. In: The biology of Drosophila (M. Demerec, ed.), p. 62–76. New York: Hafner Publishing Co. 1950
Turner, F.R., Mahowald, A.P.: Scanning electron microscopy of Drosophila melanogaster embryogenesis. II. Gastrulation and segmentation. Develop. Biol. 57, 403–416 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ellison, J.R., Howard, G.C. Non-random position of the A-T rich DNA sequences in early embryos of Drosophila virilis . Chromosoma 83, 555–561 (1981). https://doi.org/10.1007/BF00328279
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00328279