Summary
The mRNA for surface proteins from the antigenic type 51D of Paŕamecium tetraurelia was used to identify genomic DNA clones containing complementary sequences. When restriction maps were constructed for three type D genomic clones it became evident that the least two classes of paramecium DNA inserts were present, with clones λSD-3 and λSD-2 representing one class, and λSD-1 representing the other class. The transcribed regions within the inserts were mapped for all three clones. The orientation of transcription was determined for clones λSD-1 and λSD-3. S1 protection experiments revealed the ends of transcription for clone λSD-3. The entire transcribed region of this clone included about 7.5 kb of the insert. The transcribed region of λSD-1, however, appeared to be much larger than expected. In addition to their strong homologies to 51D RNA the two classes of clones (investigated in detail for λSD-1 and λSD-3) showed a strong DNA-DNA crossreaction. There was no evidence for DNA rearrangements when restriction enzyme digests of DNA from cultures of paramecia with active and inactive 51D genes were probed with DNA from these clones. It was concluded that λSD-3 corresponded to the 51D gene, and that λSD-1 represented a strongly related but unknown gene or a pseudogene.
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Communicated by B.J. Kilby
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Schmidt, H.J. Characterization and comparison of genomic DNA clones containing complementary sequences to mRNA from serotype 51D of Paramecium tetraurelia . Mol Gen Genet 208, 450–456 (1987). https://doi.org/10.1007/BF00328138
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DOI: https://doi.org/10.1007/BF00328138