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Degradation of nitriles and amides by the immobilized cells of Pseudomonas putida

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Abstract

Pseudomonas putida, capable of utilizing acetonitrile as a sole source of C and N, was immobilized in calcium alginate and the rates of degradation of nitriles, including acetonitrile, and their respective amides were studied. All the organic nitriles and amides tested were converted into NH3 and CO2.

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References

  • Adriaens, P., Kohler, H.P.E., Kohler-Staub, D. & Focht, D.D. 1989 Bacterial dehalogenation of chlorobenzoates and coculture biodegradation of 4,4′-dichlorobiphenyl. Applied and Environmental Microbiology 55, 887–892.

    Google Scholar 

  • Asano, Y., Fujishiro, K., Tani, Y. & Yamada, H. 1982 Aliphatic nitrile hydratase from Arthrobacter sp. J-1: purification and characterization. Agricultural and Biological Chemistry 46, 1165–1174.

    Google Scholar 

  • Babu, G.R.V., Wolfram, J.H. & Chapatwala, K.D. 1992 Conversion of sodium cyanide to carbon dioxide and ammonia by immobilized cells of Pseudomonas putida. Journal of Industrial Microbiology 9, 235–238.

    Google Scholar 

  • Bettmann, H. & Rehm, H.J. 1985 Continuous degradation of phenol(s) by Pseudomonas putida P8 entrapped in polyacryl amidehydrazide. Applied Microbiology and Biotechnology 22, 389–393.

    Google Scholar 

  • Bui, K., Maestracci, M., Thiery, A., Arnaud, A. & Galzy, P. 1984 A note on the enzyme action and biosynthesis of a nitrile hydratase from a Bravibacterium sp. Journal of Applied Bacteriology 57, 184–190.

    Google Scholar 

  • Chapatwala, K.D., Nawaz, M.S., Richardson, J.D. & Wolfram, J.H. 1990 Isolation and characterization of acetonitrile utilizing bacteria. Journal of Industrial Microbiology 5, 65–70.

    Google Scholar 

  • DiGeronimo, M.J. & Antoine, A.D. 1976 Metabolism of acetonitrile and propionitrile by Nocardia rhodochrocus LL1100-21. Applied and Environmental Microbiology 31, 900–906.

    Google Scholar 

  • Dwyer, S., Krumme, M.L., Boyd, S.A. & Tiedje, J.M. 1986 Kinetics of phenol biodegradation by an immobilized methanogenic consortium. Applied and Environmental Microbiology 52, 345–351.

    Google Scholar 

  • Fenn, P. & Kirk, T.K. 1979 Ligninolytic system of Phanerochaete chrysosporium: inhibition by opthalate. Archives of Microbiology 123, 307–309.

    Google Scholar 

  • Grethlein, H.E. 1985 The effect of pore size distribution on the rate of enzymatic hydrolysis of cellulosic substrates. Biotechnology 1, 155–160.

    Google Scholar 

  • Kaplan, A. 1969 The determination of urea, ammonia and urease. Methods of Biochemical Analysis 17, 311–324.

    Google Scholar 

  • Klein, J., Hackel, U. & Wagner, F. 1979 Phenol degradation by Candida tropicalis whole cells entrapped in polymeric ionic networks. In Immobilized Microbial Cells, ed Venkatsubramanin, K. pp. 101–118. Washington DC: American Chemical Society.

    Google Scholar 

  • Kunz, D.A., Nagappan, O., Avalos, J.S. & Delong, G.T. 1992 Utilization of cyanide as a nitrogenous substrate by Pseudomonas fluorescens NCIMB 11764: evidence for multiple pathways of metabolic conversion. Applied and Environmental Microbiology 58, 2022–2029.

    Google Scholar 

  • Nawaz, M.S., Chapatwala, K.D. & Wolfram, J.H. 1989 Degradation of acetonitrile by Pseudomonas putida. Applied and Environmental Microbiology 55, 2267–2274.

    Google Scholar 

  • Nawaz, M.S., Davis, J.W., Wolfram, J.H. & Chapatwala, K.D. 1991 Degradation of organic cyanides by Pseudomonas aeruginosa. Applied Biochemistry and Biotechnology 28-29, 865–875.

    Google Scholar 

  • Palleroni, N.J. 1984 Gram-negative aerobic rods and cocci. In Bergey's Manual of Systematic Bacteriology, Vol. 1, eds Krieg, N.R. & Holt, J.G. pp. 40–149. Baltimore: Williams & Wilkins.

    Google Scholar 

  • Smibert, R.M. & Krieg, N.R. 1981 General characterization. In Manual of Methods for General Bacteriology, ed Gerhardt, P. pp. 409–443. Washington DC: American Society for Microbiology.

    Google Scholar 

  • Watanabe, I., Satoh, Y., Enomoto, K., Seki, S. & Sakashita, K. 1987 Optimal conditions for the cultivation of Rhodochrocus sp. N-774 and for conversion of acrylonitrile to acrylamide by resting cells. Agricultural and Biological Chemistry 51, 3201–3206.

    Google Scholar 

  • Wood, J.M. 1982 Chlorinated hydrocarbons: oxidation in the biosphere. Environmental Sciences and Technology 16, 291a-297a.

    Google Scholar 

  • Yamada, H., Asano, Y. & Tani, Y. 1980 Microbial utilization of glutaronitrile. Journal of Fermentation Technology 58, 495–500.

    Google Scholar 

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Authors and Affiliations

  1. Division of Natural Sciences, Selma University, 36701, Selma, Alabama, USA

    K. D. Chapatwala, E. M. Hall & G. R. V. Babu

Authors
  1. K. D. Chapatwala
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  2. E. M. Hall
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  3. G. R. V. Babu
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Chapatwala, K.D., Hall, E.M. & Babu, G.R.V. Degradation of nitriles and amides by the immobilized cells of Pseudomonas putida . World Journal of Microbiology and Biotechnology 9, 483–486 (1993). https://doi.org/10.1007/BF00328038

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  • DOI: https://doi.org/10.1007/BF00328038

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