Abstract
The possible structure of human blood-group antigens, as found in cochlear hair cells of 3-day-old rats, is suggested. Data were obtained from immunocytochemical studies using 77 antibodies against the major human blood group antigens of the ABO, H, I and Lewis genetic systems. Neither the anti-A-related nor the anti-Lewis-related antibodies showed any positive immunoreaction on hair cells. In contrast, anti-B, anti-AB and anti-H antibodies displayed specific positive immunoreactive patterns on the hair cells. The results suggest that, in immature hair cells, two main glycoconjugate structures of the lactoseries are present: H type 2 antigen, which is the precursor of the B type 2 antigen, and the B type 2 antigen itself. Similar H and B carbohydrate structures have been reported in rat olfactory receptors. The type 2 glycoconjugates carrying these H and B antigens of auditive and olfactory receptors are resistant to fixation and paraffin embedding, suggesting that they might be glycoproteins. These auditive and olfactory H and B antigens must be different from the B-related antigens that are expressed by pseudo-unipolar neurons of rat posterior root ganglia, that are built from type 4 core chains, and that are destroyed by routine paraffin embedding procedures.
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Astic L, Le Pendu J, Mollicone R, Saucier D, Oriol R (1989) Cellular expression of H and B antigens in the rat olfactory system during development. J Comp Neurol 289, 386–394
Blancher A, AlSaati T, Marty Y, Delsol G (1988) Mouse monoclonal antibodies against the H and I blood group determinant. Produced by hybridomas from lymphocytes of athymic nude mice. In: Rouger P, Salmon C (eds) Monoclonal antibodies against human red blood cell and related antigens. Arnette, Paris, pp 220–223
Chester MA, Johnson U, Lundblad A, Löw B, Messeter L, Samuelsson B (1990) Proc 2nd Int Workshop on Monoclonal Antibodies against Human Red Cells and Related Antigens. April 1–4 1990, Lund, Sweden.
Chou DKH, Dodd J, Jessell TM, Costello CE (1989) Identification of α-galactose (α-Fucose)-asialo-GM1 glycolipid expressed by subsets of rat dorsal root ganglion neurons. J Biol Chem 264:3409–3415
Clausen H, Hakomori SI (1989) ABH and related histo-bloodgroup antigens. Immunochemical differences in carrier isotypes and their distribution. Vox Sang 56:1–20
Dabelsteen E, Vedtofte P, Hakomori SI, Young WW (1982) Carbohydrate chains specific for blood group antigens in differentiation of human oral epithelium. J Invest Dermatol 79:3–7
Dabelsteen E, Buschard K, Hakomori S, Young WW (1984) Pattern of distribution of blood group antigen on human epidermal cells during maturation. J Invest Dermatol 82:13–17
Dodd J, Jessell TM (1985) Lactoseries carbohydrates specify subsets of dorsal root ganglion neurons projecting to the superficial dorsal horn of rat spinal cord. J Neurosci 5:3278–3294
Feizi T (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature 314:53–57
Gil-Loyzaga P, Pujol R, Mollicone R, Dalix AM, Oriol R (1989) Appearance of B and H blood group antigens in the developing cochlear hair cells. Cell Tissue Res 257:17–21
Gil-Loyzaga P, Remezal M, Oriol R (1992) Neuronal influence on B and H human blood-group antigen expression in rat cochlear cultures. Cell Tissue Res 269:3–20
Good AH, Yau O, Lamontagne LR, Oriol R (1992) Serological and chemical specificities of twelve monoclonal anti-Lea and anti-Leb antibodies. Vox Sang 62:180–189
Hakomori S (1981) Blood group A, B, H and Le antigens of human erythrocytes: chemistry, polymorphism, and their development changes. Semin Hematol 18:39–62
Holgersson J, Breimer ME, Samuelsson BE (1992) Basic biochemistry of cell surface carbohydrates and aspects on the tissue distribution of histo-blood-group ABH and related glycosphingolipids. Acta Pathol Microbiol Immunol Scand 100 [Suppl 27]: 18–27
Kukowska-Latallo JF, Larsen RD, Nair RP, Lowe JB (1990) A cloned human cDNA determines expression of a mouse stagespecific embryonic antigen and the Lewis blood group α(1,3/1,4) fucosyltransferase. Genes Dev 4:1288–1303
Lemieux RU, Wong TC, Liao J, Kabat EA (1984) The combining site of anti-I Ma (Group 1). Mol Immunol 21:751–759
Lowe JB (1992) Molecular cloning, expression and uses of mammalian glycosyltrasnsferases. Semin Cell Biol 2:289–307
Mackenzie IC, Dabelsteen E (1987) Connective tissue influences on the expression of epithelial cell-surface antigens. Cell Tissue Res 248:137–141
Mollicone R, Bara J, Le Pendu J, Oriol R (1985a) Immunohistologic pattern of type 1 (Lea, Leb) and type 2 (X, Y, Z) blood group related antigens in the human pyloric and duodenal mucosae. Lab Invest 53:219–227
Mollicone R, Trojan J, Oriol R (1985b) Appearance of H and B antigens in primary sensory cells of the rat olfactory apparatus and inner ear. Dev Brain Res 17:275–279
Mollicone R, Davies DR, Evans B, Dalix AM, Oriol R (1986) Cellular expression and genetic control of ABH antigens in primary sensory neurons of marmoset, baboon and man. J Neuroimmunol 10:255–269
Mollicone R, Gibaud A, Francois A, Ratcliffe M, Oriol R (1990a) Acceptor specifity and tissue distribution of three human α-3-fucosyltransferases. Eur J Biochem 191:169–176
Mollicone R, Dalix AM, Gane P, Nemec M, Oriol R (1990b) Comparison of the direct binding of anti-H and anti-Y on hapten-BSA conjugates and its absorption with Synsorbs (abstract). Proc 2nd Int Workshop on Monoclonal Antibodies against Human Red Blood Cell and related Antigens, April 1–4 1990. Lund, Sweden, p 85
Mollicone R, Candelier JJ, Mennesson B, Coullin P, Venot AP, Oriol R (1992) Five specificity patterns of (1→3)-α-L-fucosyltransferase activity defined by use of synthetic oligosaccharide acceptors. Differential expression of the enzymes during human embryonic development and in adult tissues. Carbohydr Res 228:265–276
Oriol R (1987) ABH and related tissue antigens. Biochem Soc Trans 15:596–599
Oriol R (1990) Genetic control of the fucosylation of ABH precursor chains. Evidence for new epistatic interactions in different cells and tissues. J Immunogenet 17:235–245
Oriol R, Mancilla-Jimenez R (1983) Fluorescent staining nuclei and amyloid substance. Two useful properties of p-phenylenediamine. J Immunol Methods 62:185–192
Oriol R, Danilovs J, Hawkins BR (1981) A new genetic model proposing that the Se gene is a structural gene closely linked to the H gene. Am J Hum Genet 33:421–431
Oriol R, Le Mollicone R (1986) Genetics of ABO, Lewis X and related antigens. Vox Sang 51:161–171
Oriol R, Samuelsson BE, Messeter L (1990) ABH antibodies-serological behaviour and immunochemical characterization. J Immunogenet 17:279–299
Oriol R, Mollicone R, Coullin P, Dalix AM, Candelier JJ (1992) Genetic regulation of the expression of ABH and Lewis antigens in tissues. Acta Pathol Microbiol Immunol Scand 100 [Suppl 27]: 28–38
Reibel J, Dabelsteen E, Hakomori S, Young WW (1984) The distribution of blood group antigens in rodent epithelia. Cell Tissue Res 237:111–116
Rouger P, Anstee D, Salmon C (1987) Proc 1st Int Workshop on Monoclonal Antibodies against Human Red Blood Cell and Related Antigens. Blood Transfusion Immunohaematol 30:356
Sarnesto A, Köhlin T, Thurin J, Blaszczyk-Thurin M (1990) Purification of H gene-encoded β-galactoside α1→2fucosyltransferase from human serum. J Biol Chem 265:15067–15075
Sarnesto A, Köhlin T, Hindsgaul O, Thurin J, Blaszczyk-Thurin M (1992) Purification of the secretor type β-galactoside α1→2fucosyltransferase from human serum. J Biol Chem 267:2737–2744
Watkins WM (1980) Biochemistry and genetics of the ABO, Lewis and P blood group systems. Adv Hum Genet 10:1–136
Yamamoto F, Clausen H, White T, Marken J, Hakomori SI (1990) Molecular genetic basis of the histo-blood group ABO system. Nature 345:229–233
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Remezal, M., Gil-Loyzaga, P., Mollicone, R. et al. Immunocytochemical approach to the structure of human blood group B and H glycoconjugate antigens in the three days old rat cochlea. Cell Tissue Res 274, 21–26 (1993). https://doi.org/10.1007/BF00327981
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DOI: https://doi.org/10.1007/BF00327981