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Transduction of multi-copy plasmid pBR322 by bacteriophage Mu

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Summary

The temperate bacteriophage Mu transduces the 4363 bp multi-copy plasmid pBR322 at frequencies similar to those of chromosomal markers. Plasmid transducing particles contain DNA molecules of Mu DNA length. Plasmid DNA is transduced as a head-to-tail oligomer that becomes circularized in the recipient cell. The rec system of the donor strain participates in oligomer formation and the rec system of the recipient strain is required for oligomer circularization. Possible mechanisms that may explain the origin of plasmid transducing particles are discussed.

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Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Str. 1a, D-8000, München 19, Federal Republic of Germany

    Johanna Teifel-Greding

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  1. Johanna Teifel-Greding
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Communicated by H. Böhme

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Teifel-Greding, J. Transduction of multi-copy plasmid pBR322 by bacteriophage Mu. Mol Gen Genet 197, 169–174 (1984). https://doi.org/10.1007/BF00327938

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  • DOI: https://doi.org/10.1007/BF00327938

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