Summary
A revised physical map of the α-haemolysin plasmid pHly152 has been constructed. The known position of the hly genes in the restriction map of pHly152 allowed us to locate in it a direct repeat of IS elements flanking the hly genes of pHly152. These elements are IS92L, which is a derivative of the previously characterised element IS91 (1.85 kb) by insertion of a sequence of 1.2 kb, and IS92R, an element related to IS91 by a deletion of 0.7 kb and substitution of a 0.2 kb sequence of IS91 by a 1.2 kb heterologous sequence. IS92L is, in turn, flanked by an inverted repetition of sequences of 1.4 kb. These and previously published data strongly suggest that the hly genes spread at some time in evolution by means of the recombinational activity of IS91-like elements.
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References
Arthur A, Sherratt D (1979) Dissection of the transposition process: A transposon-encoded site-specific recombination system. Mol Gen Genet 175:267–274
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
Chandler M, Clerget M, Galas DJ (1982) The transposition frequency of IS1-flanked transposons is a function of their size. J Mol Biol 154:229–243
Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid. J Bacteriol 134:1141–1156
Datta N, Hedges RW (1972) Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae. J Gen Microbiol 72:349–356
De la Cruz F, Grinsted J (1982) Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1. J Bacteriol 151:222–228
De la Cruz F, Müller D, Ortiz JM, Goebel W (1980) Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups. J Bacteriol 143:825–833
De la Cruz F, Zabala JC, Ortiz JM (1983) Hemolysis determinant common to Escherichia coli strains of different 0 serotypes and origins. Infect Immun 41:881–887
Diaz-Aroca E, de la Cruz F, Zabala JC, Ortiz JM (1984) Characterization of the new insertion sequence IS91 from an alpha-hemolysin plasmid of Escherichia coli. Mol Gen Genet 193:493–499
Heffron F, Bedinger P, Champoux JJ, Falkow S (1977) Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci USA 74:702–706
Heffron F, Kostriken R, Morita C, Parker R (1981) Tn3 encodes a site-specific recombination system: Identification of essential sequences, genes, and the actual site of recombination. Cold Spring Harbor Symp Quant Biol 45:259–268
Hennecke H, Günther I, Binder F (1982) A novel cloning vector for the direct selection of recombinant DNA in Escherichia coli. Gene 19:231–234
Kleckner N (1981) Transposable elements in prokaryotes. Annu Rev Genet 15:341–404
Kleinschmidt AK (1968) Monolayer techniques in electron microscopy of nucleic acid molecules. Methods Enzymol XIIB:361–377
Kopecko DJ (1980) Specialized genetic recombination systems in bacteria: Their involvement in gene expression and evolution. Prog Mol Subcell Biol 7:135–234
Müller D, Hughes C, Goebel W (1983) Relationship between plasmid and chromosomal hemolysin determinants of Escherichia coli. J Bacteriol 153:846–851
Noegel A, Rdest U, Goebel W (1981) Determination of the functions of hemolytic plasmid pHly152 of Escherichia coli. J Bacteriol 145:233–247
Noegel A, Rdest U, Springer W, Goebel W (1979) Plasmid cistrons controlling synthesis and excretion of the exotoxin α-haemolysin of Escherichia coli. Mol Gen Genet 175:343–350
Schleif RF, Wensink PC (1981) Practical methods in molecular biology. Springer-Verlag, New York
Stuber D, Bujard H (1981) Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci USA 78:167–171
Wagner W, Vogel M, Goebel W (1983) Transport of hemolysin across the outer membrane of Escherichia coli requires two functions. J Bacteriol 154:200–210
Yang RCA, Lis J, Wu R (1979) Elution of DNA from agarose gels after electrophoresis. Methods Enzymol 68:176–182
Zabala JC, de la Cruz F, Ortiz JM (1982) Several copies of the same insertion sequence are present in alpha-hemolytic plasmids belonging to four different incompatibility groups. J Bacteriol 151:472–476
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Zabala, J.C., García-Lobo, J.M., Diaz-Aroca, E. et al. Escherichia coli alpha-haemolysin synthesis and export genes are flanked by a direct repetition of IS91-like elements. Mol Gen Genet 197, 90–97 (1984). https://doi.org/10.1007/BF00327927
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DOI: https://doi.org/10.1007/BF00327927