Summary
Resistance to aminoglycoside antibiotics in Micromonospora purpurea (the producer of gentamicin C complex), Streptomyces tenebrarius (the nebramycin producer) and Streptomyces tenjimariensis (which makes istamycin) occurs at the level of the ribosome. Reconstitution analysis has revealed, in each case, that 16S rRNA plays a critical role in determining such resistance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benveniste R, Davies J (1973) Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proc Natl Acad Sci USA 70:2276–2280
Cella R, Vining LC (1975) Resistance to streptomycin in a producting strain of Streptomyces griseus. Canad J Microbiol 21:463–472
Cundliffe E (1978) Mechanism of resistance to thiostrepton in the producing-organism, Streptomyces azureus. Nature 272:792–795
Cundliffe E (1984) Self defence in antibiotic-producing organisms. British Medical Bulletin 40:61–67
Cundliffe E, Dixon P, Stark M, Stöffler G, Ehrlich R, Stöffler-Meilicke M, Cannon M (1979) Ribosomes in thiostrepton-resistant mutants of Bacillus megaterium lacking a single 50S subunit protein. J Mol Biol 132:235–252
Davies J (1980) Aminoglycoside-aminocyclitol antibiotics and their modifying enzymes. In: Lorian V (ed) Antibiotics in laboratory medicine. The Williams & Wilkins Co, Baltimore, pp 474–489
Davies J, Davis BD (1968) Misreading of ribonucleic acid code words induced by aminoglycoside antibiotics. J Biol Chem 243:3312–3316
Davies J, Houk C, Yagisawa M, White TJ (1979) Occurence and function of aminoglycoside-modifying enzymes. In: Sebek OK, Laskin AI (eds) Genetics of industrial microorganisms. American Society for Microbiology, Washington DC, pp 166–169
Helser TL, Davies JE, Dahlberg JE (1972) Mechanism of kasugamycin resistance in Escherichia coli. Nature New Biol 235:6–9
Higgens CE, Kastner RE (1968) Nebramycin, a new broad-spectrum antibiotic complex. II. Description of Streptomyces tenebrarius. Antimicrob Agents Chemother 1967:324–331
Hotta K, Yamamoto H, Okami Y, Umezawa H (1981) Resistance mechanisms of kanamycin-, neomycin- and streptomycin-producing streptomycetes to aminoglycoside antibiotics. J Antibiot 34:1175–1182
Hotta K, Takahashi A, Okami Y, Umezawa H (1983) Relationship between antibiotic resistance and antibiotic productivity in actinomycetes which produce aminoglycoside antibiotics. J Antibiot 36:1789–1791
Kühberger R, Piepersberg W, Petzet A, Buckel P, Böck A (1979) Alteration of ribosomal protein L6 in gentamicin-resistant strains of Escherichia coli. Effects on fidelity of protein synthesis. Biochemistry 18:187–193
Lehrach H, Diamond D, Wozney JM, Boedtker H (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
Li M, Tzagoloff A, Underbrink-Lyon K, Martin NC (1982) Identification of the paromomycin-resistance mutation in the 15S rRNA gene of yeast mitochondria. J Biol Chem 257:5921–5928
Mark LG, Sigmund CD, Morgan EA (1983) Spectinomycin resistance due to a mutation in an rRNA operon of Escherichia coli. J Bacteriol 155:989–994
Murakami T, Nojiri C, Toyama H, Hayashi E, Katumata K, Anzai H, Matsuhashi Y, Yamada Y, Nagaoka K (1983) Cloning of antibiotic-resistance genes in Streptomyces. J Antibiotics 36:1305–1311
Nakano MM, Mashiko H, Ogawara H (1984) Cloning of the kanamycin resistance gene from a kanamycin-producing Streptomyces species. J Bacteriol 157:79–83
Piendl W, Böck A (1982) Ribosomal resistance in the gentamicin producer organism Micromonospora purpurea. Antimicrob Agents Chemother 22:231–236
Piwowarski JM, Shaw PD (1979) Streptomycin resistance in a streptomycin-producing microorganism. Antimicrob Agents Chemother 16:176–182
Skinner RH, Cundliffe E (1982) Dimethylation of adenine and the resistance of Streptomyces erythraeus to erythromycin. J Gen Microbiol 128:2411–2416
Skinner R, Cundliffe E, Schmidt FJ (1983) Site of action of a ribosomal RNA methylase responsible for resistance to erythromycin and other antibiotics. J Biol Chem 258:12702–12706
Tai P-C, Davis BD (1979) Triphasic concentration effects of gentamicin on activity and misreading in protein syntheis. Biochemistry 18:193–198
Thompson CJ, Gray GS (1983) Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. Proc Natl Acad Sci USA 80:5190–5194
Thompson CJ, Skinner RH, Thompson J, Ward JM, Hopwood DA, Cundliffe E (1982a) Biochemical characterization of resistance determinants cloned from antibiotic-producing streptomycetes. J Bacteriol 151:678–685
Thompson J, Schmidt F, Cundliffe E (1982b) Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton. J Biol Chem 257:7915–7917
Traub P, Nomura M (1969) Structure and function of Escherichia coli ribosomes. VI. Mechanism of assembly of 30S ribosomes studied in vitro. J Mol Biol 40:391–413
Vining LC (1979) Antibiotic tolerance in producer organisms. Adv Appl Microbiol 25:147–168
Walker MS, Walker JB (1970) Enzymatic phosphorylation of dihydrostreptobiosamine moieties of dihydrostreptomycin-(streptidino) phosphate and dihydrostreptomycin by Streptomyces extracts. J Biol Chem 245:6683–6689
Yamada T, Mizugichi Y, Nierhaus KH, Wittmann HG (1978) Resistance to viomycin conferred by RNA of either ribosomal subunit. Nature 275:460–461
Yamamoto H, Hotta K, Okami Y, Umezawa H (1981a) Self-resistance of a Streptomyces which produces istamycins. J Antibiot 34:824–829
Yamamoto H, Hotta K, Okami Y, Umezawa H (1981b) Ribosomal resistance of an istamycin producer, Streptomyces tenjimariensis, to aminoglycoside antibiotics. Biochem Biophys Res Commun 100:1396–1401
Yamamoto H, Hotta K, Okami Y, Umezawa H (1982) Mechanism of resistance to aminoglycoside antibiotics in nebramycin producting Streptomyces tenebrarius. J Antibiot 35:1020–1025
Author information
Authors and Affiliations
Additional information
Communicated by E. Bautz
Rights and permissions
About this article
Cite this article
Piendl, W., Böck, A. & Cundliffe, E. Involvement of 16S ribosomal RNA in resistance of the aminoglycoside-producers Streptomyces tenjimariensis, Streptomyces tenebrarius and Micromonospora purpurea . Mol Gen Genet 197, 24–29 (1984). https://doi.org/10.1007/BF00327918
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00327918