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Production of pectin lyase by Penicillium griseoroseum as a function of the inoculum and culture conditions

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Abstract

Optimum activity of an extracellular pectin lyase produced by Penicillium griseoroseum in submerged culture was after 120 h using 0.1% (w/v) citrus pectin as substrate. Sucrose at 0.1% (w/v) stimulated enzyme production and citrus pectin gave the highest activity of enzyme per unit growth.

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References

  • Alana, A., Hernando, F., Moragues, M.D., Dominguez, J.B., Llama, M.J. & Serra, J.L. 1989 Pectin lyase production by a Penicillium italicum strain. Applied and Environmental Microbiology 55, 1612–1616.

    Google Scholar 

  • Albersheim, P. 1966 Pectin lyase from fungi. Methods in Enzymology 8, 628–635.

    Google Scholar 

  • Albersheim, P. & Killias, U. 1962 Studies relating to the purification and properties of pectin transeliminase. Archives of Biochemistry and Biophysics 97, 107–115.

    Google Scholar 

  • El-Refai, A.A., Metwalli, S.M. & El-Sebaiy, L.A. 1984 Influence of pH, inoculum concentration, aeration and growth period on production of pectolytic enzymes by Aspergillus awamori 16. Chemische Mikrobiologie und Technologie der Lebensmittel 8, 115–117.

    Google Scholar 

  • Ishii, S. & Yokotsuka, T. 1971 Pectin trans-eliminase with fruit juice clarifying activity. Journal of Agricultural and Food Chemistry 19, 958–961.

    Google Scholar 

  • Lobanok, A.G., Sapunova, L.I., Oparina, A.A. & Mikhailova, R.V. 1983 Production of pectin depolymerases by Aspergillus alliaceus as a function of the state and amount of the inoculum. Applied Biochemistry and Microbiology (USSR) 19, 582–586.

    Google Scholar 

  • Olutiola, P.O. & Akintunde, O.A. 1979 Pectin lyase and pectin methylesterase production by Penicillium citrinum. Transactions of the British Mycological Society 72, 49–55.

    Google Scholar 

  • Schlemmer, A.F., Ware, C.F. & Keen, N.T. 1987 Purification and characterization of a pectin lyase produced by Pseudomonas fluorescens W51. Journal of Bacteriology 169, 4493–4498.

    Google Scholar 

  • Spalding, D.H. & Abdul-Baki, A.A. 1972 In vitro and in vivo production of pectin lyase by Penicillium expansum. Phytopathology 63, 231–235.

    Google Scholar 

  • Strzelczyk, E., Dahm, P.I. & Kowalski, S. 1987 The influence of pH and temperature on cellulolytic and pectolytic activity of Cylindrocarpon destructans (Zins.) Scholt. Journal of Phytopathology 120, 97–103.

    Google Scholar 

  • Tuttobello, R. & Mill, P.J. 1961 The pectic enzymes of Aspergillus niger. 1. The production of active mixtures of pectic enzymes. Biochemical Journal 79, 51–57.

    Google Scholar 

  • Zetelaki-Horvath, K. & Thien, X.N. 1985 Kinetic analysis of pectin lyase synthesis of an Aspergillus strain. Acta Alimentaria 14, 165–172.

    Google Scholar 

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Brumano, M.H.N., Coelho, J.L.C., Araújo, E.F. et al. Production of pectin lyase by Penicillium griseoroseum as a function of the inoculum and culture conditions. World J Microbiol Biotechnol 9, 225–228 (1993). https://doi.org/10.1007/BF00327843

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  • DOI: https://doi.org/10.1007/BF00327843

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