Abstract
β-Fructofuranosidase from Aspergillus japonicus MU-2, which produces fructo-oligosaccharides (1-kestose: O-β-D-fructofuranosyl-(2 → 1)-β-D-fructofuranosyl α-D-glucopyranoside); and nystose: O-β-D-fructofuranosyl-(2 → 1)-β-D-fructofuranosyl-(2 → 1)-β-D-fructofuranosyl α-D-glucopyranoside) from sucrose, was immobilized, covalently with glutaraldehyde onto alkylamine porous silica, at high efficiency (64%). Optimum pore diameter of porous silica for immobilization of the enzyme was 91.7 nm. After immobilization, the enzyme's stabilities to temperature, metal ions and proteolysis were improved, while its optimum pH and temperature were unchanged. The highest efficiency of continuous production of fructo-oligosaccharides (more than 60%), using a column packed with the immobilized enzyme, was obtained at 40% to 50% (w/v) sucrose. The half-life of the column during long-term continuous operation at 55°C was 29 days.
Similar content being viewed by others
References
Gisin, B.F. 1972 The monitoring of reaction in solid-phase peptide synthesis with picric acid. Analytica Chimica Acta 58, 248–249.
Hayashi, S., Hayashi, T., Kinoshita, J., Takasaki, Y. & Imada, K. 1992a Immobilization of β-fructofuranosidase from Aureobasidium sp. ATCC 20524 on porous silica. Journal of Industrial Microbiology 9, 247–250.
Hayashi, S., Ito, K., Nonoguchi, M., Takasaki, Y. & Imada, K. 1991a Immobilization of a fructosyl-transferring enzyme from Aureobasidium sp. on shirasu porous glass. Journal of Fermentation and Bioengineering 72, 68–70.
Hayashi, S., Kinoshita, J., Nonoguchi, M., Takasaki, Y. & Imada, K. 1991b Continuous production of 1-kestose by β-fructofuranosidase immobilized on shirasu porous glass. Biotechnology Letters 13, 395–398.
Hayashi, S., Matsuzaki, K., Takasaki, Y., Ueno, H. & Imada, K. 1992b Production of β-fructofuranosidase by Aspergillus japonicus. World Journal of Microbiology and Biotechnology 8, 155–159.
Hayashi, S., Matsuzaki, K., Takasaki, Y., Ueno, H. & Imada, K. 1992c Purification and properties of β-fructofuranosidase from Aspergillus japonicus. World Journal of Microbiology and Biotechnology 8, 276–279.
Koboli, H. & Reilly, P.J. 1980 Immobilization and properties of Leuconostoc mesenteroides dextransucrase. Biotechnology and Bioengineering 22, 1055–1069.
Lee, D.D., Lee, Y.Y., Reilly, P.J., CollinsJr, E.V. & Tsao, G.T. 1976 Pilot plant production of glucose with glucoamylase immobilized to porous silica. Biotechnology and Bioengineering 18, 253–267.
Lomako, O.V., Menyailova, I.I., Nakhapetyan, L.A., Nikitin, Y. & Kiselev, A.V. 1982 Immobilization of glucoamylase on porous silicas. Enzyme and Microbial Technology 4, 89–92.
Mozaffar, Z., Nakanishi, K. & Matsumoto, R. 1986 Continuous production of galacto-oligosaccharides from lactose using immobilized β-galactosidase from Bacillus circulans. Applied Microbiology and Biotechnology 25, 224–228.
Weetall, H.H. 1977 Covalent coupling methods for inorganic support materials. Methods in Enzymology 44, 134–143.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hayashi, S., Matsuzaki, K., Inomata, Y. et al. Properties of Aspergillus japonicus β-fructofuranosidase immobilized on porous silica. World J Microbiol Biotechnol 9, 216–220 (1993). https://doi.org/10.1007/BF00327841
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00327841