Abstract
A strain of Rhizopus stolonifer produced a high activity of extracellular DNAase when grown on YPG (yeast extract peptone glucose) medium. The source of peptone had a marked effect on the enzyme yield and only one peptone (i.e. from Sarabhai M. Chemicals Ltd, India) supported enzyme production. Maximum enzyme activity (88 U/ml) was obtained after 4 days' growth under submerged conditions in YPG medium containing 100 μM Mn2+, Co2+ or Mg2+, and glucose as the sole carbon source. The unpurified enzyme was optimally active at pH 7.5 and 45°C. It had a higher activity with sonicated and heat-denatured DNA than native DNA.
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Apte, P.S., Deshpande, M.V. & Shankar, V. Optimization of medium for extracellular nuclease formation from Rhizopus stolonifer . World J Microbiol Biotechnol 9, 205–209 (1993). https://doi.org/10.1007/BF00327838
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DOI: https://doi.org/10.1007/BF00327838