Abstract
During the production of pectinases by a strain of Aspergillus niger isolated from rotten lemons, methanol was liberated into the medium due to the cleavage of the pectin molecule used as the carbon source. The methanol was subsequently consumed by the microorganism but neither the synthesis nor the activity of pectinesterase and polygalacturonase was affected. Although not studied in detail, the mechanism involved in the utilization of methanol is similar to that described for methylotrophic yeasts.
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References
Bystrykh, L.V., DeKoning, W. & Harder, W. 1990 Dihydroxyacetone synthase from Candida boidinii KD1. Methods in Enzymology 188, 435–441.
Cabello Velazco, A., Ruiz, S. & Orihuela Alcocer, M. 1982 Producción de pectinasas bacterianas utilizando pulpa de café como sustrato. Revista Latinoamericana de Microbiología 24, 172–179.
Kertesz, Z.I. 1955 Pectic enzymes. Methods in Enzymology 1, 158–162.
Krug, E.L., Lim, H. & Tsao, G. 1979 Single cell protein from C1 compounds. Annual Reports on Fermentation Processes 3, 141–195.
Litchfield, J. 1979 Microbial Technology. In Microbial Processes, Vol. 1, eds Peppler, H.J. & Perlman, D. pp. 100–107. New York: Academic Press.
Maldonado, M.C., Navarro, A.R. & Callieri, D.A. 1986 Production of pectinases by Aspergillus sp using differently pretreated lemon peel as the carbon source. Biotechnology Letters 8, 501–504.
Maldonado, M.C., Strasser de Saad, A.M. & Callieri, D.A.S. 1989a Some regulatory aspects of the synthesis of polygalacturonase and pectinesterase by Aspergillus niger sp. Sciences des Aliments 9, 101–110.
Maldonado, M.C., Strasser de Saad, A.M. & Callieri, D.A.S. 1989b Catabolite repression of the synthesis of inducible polygalacturonase and pectinesterase by Aspergillus niger sp. Current Microbiology 18, 303–306.
Miller, G.L. 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426–428.
Rose, Z.B. & Racker, E. 1966 Formaldehyde dehydrogenase. Methods in Enzymology 10, 357–358.
Sahm, H. 1977 Metabolism of methanol by yeast. In Advances in Biochemical Engineering, Vol. 6, eds Ghose, T.K. Fiechter, A. & Blakebrough, N. pp. 77–103. New York: Springer Verlag.
Tani, Y., Kato, N. & Yamada, H. 1978 Utilization of methanol by yeast. Advances in Applied Microbiology 24, 165–186.
Yamada, H., Tani, Y., Nishizawa, T., Kato, N., & Sakazawa, C. 1981 Methanol utilization by yeast Kloeckera sp no. 2201: the first enzyme in the assimilation pathway. In Advances in Biotechnology, Vol. 2, eds Moo-Young, M. & Robinson, C. pp. 407–412. New York: Pergamon Press.
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Maldonado, M.C., Strasser de Saad, A.M. & Callieri, D.A.S. Methanol utilization by a strain of Aspergillus niger: influence on the synthesis and activity of pectinases. World J Microbiol Biotechnol 9, 202–204 (1993). https://doi.org/10.1007/BF00327837
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DOI: https://doi.org/10.1007/BF00327837