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World Journal of Microbiology and Biotechnology

, Volume 12, Issue 1, pp 107–108 | Cite as

Short communication: Methylparathion degradation by Pseudomonas sp. A3 immobilized in sodium alginate beads

  • M. P. Ramanathan
  • D. Lalithakumari
Short Communication

Abstract

An organophosphate-degrading soil isolate of Pseudomonas sp. A3, immobilized at 5% (wet wt/v) cell mass in 3% (w/v) sodium alginate beads, detoxified 99% of 1 mm methylparathion in 48 h. The beads were re-usable for five batches, the sixth batch only giving 73% methylparathion removal.

Key words

Cell immobilization methylparathion Pseudomonas sp. 

References

  1. Caldwell, C.R. & Raushel, F.M. 1991 Detoxification of organophosphate pesticides using an immobilized phosphotriesterase from Pseudomonas diminuta. Biotechnology and Bioengineering 37, 103–109.Google Scholar
  2. Saunders, J.R., Docherty, A. & Humphreys, G.O. 1984 Transformation of bacteria by plasmid DNA. Methods in Microbiology 17, 61–95.Google Scholar
  3. Siddharamappa, R., Rajaram, K.P. & Sethunathan, N. 1973 Degradation of parathion by bacteria isolated from flooded soil. Applied Microbiology 26, 846–849.Google Scholar

Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  • M. P. Ramanathan
    • 1
  • D. Lalithakumari
    • 1
  1. 1.Pesticide Research Laboratory. Centre for Advanced Studies in BotanyUniversity of MadrasMadrasIndia

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