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Chemically defined collateral projections from the pons to the central nucleus of the amygdala and hypothalamic paraventricular nucleus in the rat

Cell and Tissue Research volume 277, pages 289–295 (1994)Cite this article

Abstract

Triple fluorescence labelling was employed to reveal the distribution of chemically identified neurons within the pontine laterodorsal tegmental nucleus and dorsal raphe nucleus which supply branching collateral input to the central nucleus of the amygdala and hypothalamic paraventricular nucleus. The chemical identity of neurons in the laterodorsal tegmental nucleus was revealed by immunocytochemical detection of choline-acetyltransferase or substance P; in the dorsal raphe nucleus, the chemical content of the neurons was revealed with antibody recognizing serotonin. The projections were defined by injections of two retrograde tracers, rhodamine-and fluorescein-labelled latex microspheres, in the central nucleus of the amygdala and paraventricular nucleus, respectively. Neurons projecting to both the central nucleus of the amygdala and the paraventricular nucleus were distributed primarily within the caudal extensions of the laterodorsal tegmental nucleus and dorsal raphe nucleus. Approximately 11% and 7% of the labelled cells in the laterodorsal tegmental nucleus and dorsal raphe nucleus projected via branching collaterals to the paraventricular nucleus and central nucleus of the amygdala. About half of these neurons in the laterodorsal tegmental nucleus were cholinergic, and one-third were substance-P-ergic; in the dorsal raphe nucleus, approximately half of the neurons containing both retrograde tracers were serotonergic. These results indicate that pontine neurons may simultaneously transmit signals to the central nucleus of the amygdala and paraventricular nucleus and that several different neuroactive substances are found in the neurons participating in these pathways. This coordinated signalling may lead to synchronized responses of the central nucleus of the amygdala and paraventricular nucleus for the maintenance of homeostasis. Interactions between different neuroactive substances at the target site may serve to modulate the responses of individual neurons.

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Affiliations

  1. Department of Anatomy and Cell Biology, Faculty of Medicine, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada

    Theodor Petrov & Teresa L. Krukoff

  2. Department of Medicine, Faculty of Medicine, University of Alberta, TGG 2H7, Edmonton, Alberta, Canada

    Jack H. Jhamandas

Authors
  1. Theodor Petrov
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  2. Teresa L. Krukoff
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  3. Jack H. Jhamandas
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Petrov, T., Krukoff, T.L. & Jhamandas, J.H. Chemically defined collateral projections from the pons to the central nucleus of the amygdala and hypothalamic paraventricular nucleus in the rat. Cell Tissue Res 277, 289–295 (1994). https://doi.org/10.1007/BF00327776

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  • DOI: https://doi.org/10.1007/BF00327776

Key words

  • Serotonin
  • Substance P
  • Choline-acetyltransferase
  • Retrograde tracers
  • Immunocytochemistry
  • Laterodorsal tegmental nucleus
  • Dorsal raphe nucleus
  • Rat (Sprague Dawley)