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Peptidergic innervation of rat lymphoid tissue and lung: Relation to mast cells and sensitivity to capsaicin and immunization

Cell and Tissue Research volume 262, pages 125–133 (1990)Cite this article

Summary

The peptidergic innervation of lymphoid tissue and the lung in relation to mast cells was studied in rat. The sensitivity of neuropeptide-containing nerves to capsaicin treatment and immunization was also examined. Measurements of the content of neurokinin A and calcitonin gene-related peptide revealed that the lung contained the highest content of both neuropeptides; lymph nodes had intermediate levels, whereas the spleen had the lowest content. Immuhohistochemistry showed that the calcitonin gene-related peptide- and neurokinin A-immunoreactive nerves in lymph nodes were mainly found around blood vessels, whereas in the lung the nerves were present within the lining respiratory epithelium, bronchial smooth muscle, around blood vessels and close to lymphoid aggregates. Combined immunohistochemistry for serotonin (5-hydroxytryptamine), as a marker for mast cells, and tachykinins or calcitonin gene-related peptide revealed that a close association was often present between the nerves and 5-hydroxytryptamine-positive cells in the bronchi of the lung, while 5-hydroxytryptamine-positive cells were not observed in lymph nodes. The neurokinin A and calcitonin gene-related peptide content in lymph nodes, spleen and lung, but not the content of neuropeptide Y, was markedly decreased by capsaicin treatment, suggesting a sensory origin for the two former peptides. Aerosol immunization increased the levels of calcitonin gene-related peptide in the lung, whereas the content in mediastinal lymph nodes was not affected. These data demonstrate a peptidergic innervation mainly of blood vessels in lymphoid tissue and a close relation between sensory nerves and mast cells as well as lymphoid aggregates in the bronchi of the lung. This further suggests that the sensory innervation of lymph nodes is mainly related to regulation of vascular tone and lymph flow. Furthermore, at the site of immunization, i.e., in the airway mucosa, sensory nerve mediators may interact both with mast cells and lymphoid cells.

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Affiliations

  1. Department of Allergy and Immunology, Pharmacia Diagnostics AB, Uppsala, Sweden

    G. Nilsson, K. Alving & S. Ahlstedt

  2. Department of Immunology, Uppsala University, Uppsala, Sweden

    G. Nilsson

  3. Department of Pharmacology, Karolinska Institute, Stockholm, Sweden

    K. Alving & J. M. Lundberg

  4. Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden

    T. Hökfelt

Authors
  1. G. Nilsson
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  2. K. Alving
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  3. S. Ahlstedt
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  4. T. Hökfelt
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  5. J. M. Lundberg
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Nilsson, G., Alving, K., Ahlstedt, S. et al. Peptidergic innervation of rat lymphoid tissue and lung: Relation to mast cells and sensitivity to capsaicin and immunization. Cell Tissue Res 262, 125–133 (1990). https://doi.org/10.1007/BF00327753

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  • DOI: https://doi.org/10.1007/BF00327753

Key words

  • Neurokinin A
  • Calcitonin gene-related peptide
  • Neuropeptide Y
  • Capsaicin
  • Mast cells
  • Lung
  • Lymph nodes
  • Rat (BN x Wi/Fu)