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Intragranular co-storage of neuropeptide Y and arginine vasopressin in the paraventricular magnocellular neurons of the rat hypothalamus

Cell and Tissue Research volume 262, pages 47–52 (1990)Cite this article

Summary

Certain populations of arginine vasopressin (AVP) neurons in the magnocellular paraventricular nucleus became immunoreactive for neuropeptide Y (NPY) when rats were treated with colchicine or monosodium glutamate (MSG). The co-storage of these peptides was examined by empooying a post-embedding electron-microscopic immunohistochemistry technique using goldlabeled antibodies to the two peptides. In colchicinetreated rats, the neuronal perikarya contained numerous secretory granules showing co-storage of the two peptides. The cells of the MSG-treated rats were characterized by having well-developed Golgi bodies with the granular structures also co-storing the two peptides, although the secretory granules in the perikarya were rather fewer than in the colchicine-treated rats. It is concluded that the destruction of the arcuate nucleus by MSG-treatment may potentiate the synthesis of NPY in AVP neurons, the synthesis of which is latent in intact animals.

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Affiliations

  1. Department of Anatomy, School of Medicine, The University of Tokushima, Tokushima, Japan

    Yasuaki Kagotani, Setsuji Hisano, Yoshihiro Tsuruo & Shigeo Daikoku

  2. International Center for Medical Research, Kobe University School of Medicine, Kobe, Japan

    Yasuhiko Okimura & Kazuo Chihara

Authors
  1. Yasuaki Kagotani
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  2. Setsuji Hisano
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  3. Yoshihiro Tsuruo
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  4. Shigeo Daikoku
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  5. Yasuhiko Okimura
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  6. Kazuo Chihara
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Kagotani, Y., Hisano, S., Tsuruo, Y. et al. Intragranular co-storage of neuropeptide Y and arginine vasopressin in the paraventricular magnocellular neurons of the rat hypothalamus. Cell Tissue Res 262, 47–52 (1990). https://doi.org/10.1007/BF00327744

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  • DOI: https://doi.org/10.1007/BF00327744

Key words

  • Neuropeptide Y
  • Arginine vasopressin
  • Co-storage
  • Immunohistochemistry
  • Electron microscopy
  • Paraventricular nucleus
  • Rat (Sprague-Dawley)