Summary
DNA coding for ribosomal RNA in Podospora anserina has been cloned and was found as a tandemly repeated 8.3 kb sequence. The cloned rDNA was characterized by restriction endonuclease mapping. The location of 5.8S, 18S and 28S rRNA coding regions was established by DNA-RNA hybridization and S1 nuclease mapping. The organization of P. anserina rRNA genes is similar to that of Neurospora crassa and Aspergillus nidulans. The rDNA unit does not contain the sequence coding for 5S RNA.
References
Bégueret J, Perrot M, Crouzet M (1977) Ribosomal proteins in the fungus Podospora anserina: evidence for an electrophoretically altered 60S protein in a cycloheximide resistant mutant. Mol Gen Genet 156:141–144
Bell GI, De Gennaro LJ, Gelfand DH, Bishop RJ, Valenzuela P, Rutter WJ (1977) Ribosomal RNA genes of Saccharomyces cerevisiae. J Biol Chem 252:8118–8125
Berk AJ, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732
Borsuk PA, Nagiec MM, Stepien PP, Bartnik E (1982) Organization of the ribosomal RNA gene cluster in Aspergillus nidulans. Gene 17:147–152
Cihlar RL, Sypherd PS (1980) The organization of the ribosomal RNA genes in the Fungus Mucor racemosus. Nucl Acids Res 8:793–804
Cox RA, Peden K (1979) A study of the organization of the ribosomal ribonucleic acid gene cluster of Neurospora crassa by means of restriction endonuclease analysis and cloning in bacteriophage lambda. Mol Gen Genet 174:17–24
Cramer JH, Farrelly FW, Bartniz JT, Rownd RH (1977) Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA. Mol Gen Genet 148:233–241
Crouzet M, Bégueret J (1980) A new mutant form of the ribosomal protein L21 in the fungus Podospora anserina. Identification of the structural gene for this protein. Mol Gen Genet 180:177–183
Crouzet M, Bégueret J (1982) Altered ribosomal proteins in emetine resistant strains in the fungus Podospora anserina. Curr Genet 6:39–45
Free SJ, Rice PW, Metzenberg RL (1979) Arrangement of the genes coding for ribosomal ribonucleic acids in N. crassa. J Bact 137:1219–1226
Krumlauf R, Marzluf GA (1980) Genome organization and characterization of the repetitive and inverted repeat DNA sequences in Neurospora crassa. J Biol Chem 255:1138–1145
Loening UE (1968) Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol 38:355–365
Philippsen P, Thomas M, Kramer RA, Davis RW (1977) Unique arrangement of coding sequences for 5S, 5.8S, 18S and 25S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis. J Mol Biol 123:387–404
Rozek CE, Timberlake WE (1979) Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexualis. Nucl Acids Res 7:1567–1578
Schweizer E, Mac Kechnie C, Halvorson HO (1969) The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol 40:261–277
Selker EU, Yanofsky C (1981) Dispersed 5S RNA genes in N. crassa: structure expression and evolution. Cell 24:819–828
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Author information
Authors and Affiliations
Additional information
Communicated by W. Gajewski
Rights and permissions
About this article
Cite this article
Labat, N., Perrot, M. & Bégueret, J. Cloning and characterization of the rDNA repeat unit of Podospora anserina . Molec Gen Genet 199, 154–157 (1985). https://doi.org/10.1007/BF00327526
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00327526