Summary
We have constructed a restriction map for the genome of bacteriophage MX-8 from Myxococcus xanthus using the enzymes PvuII, MboI, and EcoRI. The phage genome size, as determined by restriction analysis, is 51.7±0.6 Kb. Double digestions, redigestions of isolated fragments, and crossed-contact hybridization of partial digestion products show that the restriction map is circular. Restriction analysis and Southern hybridization show that the phage DNA molecules are packaged sequentially from a concatemer starting from a specific site which we have mapped. The DNA molecules have an average terminal redundancy of approximately 8% and are circularly permuted over at least 40% of the genome.
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Communicated by W. Arber
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Stellwag, E., Fink, J.M. & Zissler, J. Physical characterization of the genome of the Myxococcus xanthus bacteriophage MX-8. Molec Gen Genet 199, 123–132 (1985). https://doi.org/10.1007/BF00327521
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DOI: https://doi.org/10.1007/BF00327521