Summary
It has been proposed that the mutation fixation processes stimulated by SOS induction result from an induced infidelity of DNA replication (Radman 1974). The aim of this study was to determine if mutator mutations in the E. coli DNA polymerase III might affect UV-induced mutagenesis.
Using a phage λ mutation assay which can discriminate between targeted and untargeted mutations, we show that the polC74 mutator mutation (Sevastopoulos and Glaser 1977) primarily affects untargeted mutagenesis, which occurs in a recA1 genetic background and is amplified in the recA + genetic background. The polC74 mutation also increases the UV-induced mutagenesis of the bacterial chromosome. These results suggest that DNA polymerase III is involved in the process of UV-induced mutagenesis in E. coli.
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References
Brandenburger A, Godson GN, Radman M, Glickman BW, van Sluis CA, Doubleday OP (1981) Radiation-induced base substitution mutagenesis in single stranded phage M13. Nature 294:180–182
Bridges BA, Mottershead RP, Sedgwick SG (1976) Mutagenic DNa repair in E. coli. III. Requirement for a function of DNA polymerase III in ultraviolet light mutagenesis. Mol Gen Genet 144:53–58
Bridges BA, Woodgate R (1984) Mutagenic repair in E. coli. X. The umuC gene product may be required for replication past pyrimidine dimers but not for the coding error in UV-mutagenesis. Mol Gen Genet 196:364–366
Caillet-Fauquet P, Defais M (1977) Phage yield during W-reactivation of bacteriophage λ. Mutat Res 45:161–167
Caillet-Fauquet P, Defais M, Radman M (1977) Molecular mechanisms of induced mutagenesis. Replication in vivo of bacteriophage ϕX174 single-stranded, ultraviolet light-irradiated DNA in intact and irradiated host cells. J Mol Biol 117:95–112
Caillet-Fauquet P, Maenhaut-Michel G (1982) Increased detection of SOS mutator effect in E. coli mutants deficient in mismatch correction. Biochimie 64:661–663
Caillet-Fauquet P, Maenhaut-Michel G, Radman M (1984) SOS mutator effect in E. coli mutants deficient in mismatch correction. EMBO J 3:707–712
Coulondre C, Miller JH (1977) Genetic studies of the lac repressor. IV. Mutagen specificity in the lacI gene of E. coli. J Mol Biol 117:577–606
Defais M (1983) Role of the E. coli umuC gene product in the repair of single-stranded DNA phage. Mol Gen Genet 192: 509–511
Defais M, Fauquet P, Radman M, Errara M (1971) Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems. Virology 43:495–503
Howard-Flanders P, Boyce RP (1966) DNA repair and genetic recombination: studies on mutants of E. coli defective in these processes. Radiat Res (Suppl) 6:156–184
Ichikawa-Ryo H, Kondo S (1975) Indirect mutagenesis in phage λ by ultraviolet preirradiation of host bacteria. J Mol Biol 97:77–92
Jacob F (1954) Mutation d'un bactériophage induite par l'irradiation des seules bacteries hotes avant l'infection. CR Acad Sci Paris 238:732–734
Kaiser AD (1955) A genetic study of the temperate coliphage. Virology 1:424–443
Kato T, Rothman RH, Clark AJ (1977) Analysis of the role of recombination and repair in mutagenesis of E. coli by UV irradiation. Genetics 87:1–18
Kato T, Shinoura Y (1977) Isolation and characterization of mutants of E. coli deficient in induction of mutations by UV light. Mol Gen Genet 156:121–131
Kornberg T, Gefter ML (1971) A second polymerase activity distinct from polymerases I and II has been observed in PolA1 extracts; its properties are described. Proc Natl Acad Sci USA 68:761–764
LeClerc JE, Istock NL (1982) Specificity of UV mutagenesis in the lac promoter of M13 lac hybride phage DNA. Nature 297:596–598
Maenhaut-Michel G, Caillet-Fauquet P (1984) The effect of umuC mutations on targeted and untargeted ultraviolet mutagenesis in bacteriophage λ. J Mol Biol 177:181–187
Quillardet P, Devoret R (1982) Damaged-site independent mutagenesis of phage λ produced by inducible error-prone repair. Biochimie 64:789–796
Radman M (1974) Phenomenology of an inducible mutagenic DNA repair pathway in E. coli SOS repair hypothesis. In: Prakash L, Sherman F, Miller MW, Lawrence CW, Taber HW (eds) Molecular and environmental aspects of mutagenesis. 6th Rochester Conference on Environmental Toxicity, C.C. Thomas, Springfield II, USA, pp 128–142
Salles B, Paoletti C (1983) Control of UV induction of RecA protein. Proc Natl Acad Sci USA 80:65–69
Scheuermann R, Schuman T, Burgers PMJ, Lu C, Echols M (1983) Identification of the ε-subunit of E. coli DNA polymerase III holoenzyme as the dnaQ gene product: a fidelity subunit for DNA replication. Proc Natl Acad Sci USA 80:7085–7089
Sevastopoulos CG, Glaser DA (1977) Mutator action by E. coli strains carrying dnaE mutations. Proc Natl Acad Sci USA 74:3947–3950
Shizuya H, Livingston DM, Richardson C (1974) Isolation of a specialized transducing bacteriophage lambda carrying the polC locus of E. coli. Proc Natl Acad Sci USA 71:2614–2617
Skalka AM (1977) DNA replication-Bacteriophage λ. Curr Top Microbiol Immunol 78:201–237
Steinborn G (1978) Uvm mutants of E. coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis. Mol Gen Genet 165:87–93
Walker GC (1984) Mutagenesis and inducible responses to deoxyribonucleic acid damage in E. coli. Microbiol Rev 48:60–93
Walker GC, Dobson PP (1979) Mutagenesis and repair deficiencies of E. coli umuC mutants are suppressed by the plasmid pKM101. Mol Gen Genet 172:17–24
Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in E. coli. Bacteriol Rev 40:869–967
Witkin EM, Kogoma T (1984) Involvement of the activated form of RecA protein in SOS mutagenesis and stable DNA replication in E. coli. Proc Natl Acad Sci USA 81:7539–7543
Witkin EM, Wermundsen IE (1978) Targeted and untargeted mutagenesis by various inducers of SOS functions in E. coli. Cold Spring Harbor Symp Quant Biol 43:881–886
Wood RD, Hutchinson F (1984) Non-targeted mutagenesis of unirradiated λ phage in E. coli host cells irradiated with U.V. light. J Mol Biol 173:293–305
Wood RD, Skopek TR, Hutchinson F (1984) Changes in DNa base sequence induced by targeted mutagenesis of λ phage by ultraviolet light. J Mol Biol 173:273–291
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Brotcorne-Lannoye, A., Maenhaut-Michel, G. & Radman, M. Involvement of DNA polymerase III in UV-induced mutagenesis of bacteriophage lambda. Molec Gen Genet 199, 64–69 (1985). https://doi.org/10.1007/BF00327511
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DOI: https://doi.org/10.1007/BF00327511