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Physical mapping of the gene for aminopeptidase N in Escherichia coli K12

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Summary

The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF and asnS genes are contiguous and pepN is separated from asnS by a DNA fragment of about 1.6 kb.

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References

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Authors and Affiliations

  1. Laboratoire de Chimie Bactérienne, C.N.R.S., B.P. 71, 13277, Marseille Cedex 9, (France)

    M. Bally, M. Murgier & A. Lazdunski

  2. Department of Molecular Cell Biology, Section Microbiology, State University, Transitorium 3, Padualaan 8, 3584 CH, Utrecht, (The Netherlands)

    J. Tommassen

Authors
  1. M. Bally
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  2. M. Murgier
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  3. J. Tommassen
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  4. A. Lazdunski
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Communicated by H. Böhme

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Bally, M., Murgier, M., Tommassen, J. et al. Physical mapping of the gene for aminopeptidase N in Escherichia coli K12. Molec. Gen. Genet. 193, 190–191 (1984). https://doi.org/10.1007/BF00327436

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  • DOI: https://doi.org/10.1007/BF00327436

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