Summary
Hybrid enzymes composed of subunits of oligomeric isozymes present in different subcellular compartments have never been observed in vivo, even though their precursors are commonly synthesized in the same cytosol. To investigate this problem, we examined in plants the dimeric enzyme triose phosphate isomerase, which is present as two nuclear-coded isozymes, one in the plastids and the other in the cytosol. We dissociated both purified isozymes from several unrelated species into subunits, and then reassociated the unlike subunits into active hybrid enzymes. Since such hybrid enzymes are not observed in vivo, but can be formed in vitro, a specific mechanism must prevent either their formation or their accumulation in the cell. Possible mechanisms are discussed.
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References
Alber T, Kawasaki G (1982) Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae. J Mol App Genet 1:419–434
Baum JA, Scandalios JG (1982) Multiple genes controlling superoxide dismutase expression in maize. J Hered 73:95–100
Bukowiecki AC, Anderson LE (1974) Multiple forms of alsolase and triose phosphate isomerase in diverse plant species. Plant Sci Lett 3:381–386
Cerff R, Kloppstech K (1982) Structural diversity and differential control of mRNA coding for angiosperm glyceraldehyde-3-phosphate dehydrogenases. Proc Natl Acad Sci USA 79:7624–7628
Chilson OP, Kitto B, Priddle J, Kaplan NO (1966) Reversible inactivation of dehydrogenases. J Biol Chem 241:2431–2445
Chua NH, Schmidt GH (1979) Transport of proteins into mitochondria and chloroplasts. J Cell Biol 81:461–483
David BD, Tai PC (1980) The mechanism of protein secretion across membranes. Nature (London) 283:433–438
Edwards YH, Hopkins DA (1977) Developmental changes in electrophoretic patterns of human enzymes and other proteins. In Isozymes: current topics in biological and medical research 1:19–78 (A. Liss Inc. N.Y.)
Ellis RJ (1981) Chloroplast proteins: synthesis, transport, and assembly. Ann Rev Plant Physiol 32:111–137
Gallez GP, Gottlieb LD (1982) Genetic evidence for the hybrid origin of the diploid plant Stephanomeria diegensis. Evolution 36:1158–1167
Gottlieb LD (1982) Conservation and duplication of isozymes in plants. Science 216:373–380
Harmey MH, Neupert W (1979) Biosynthesis of mitochondrial citrate synthase in Neurospora crassa. FEBS Lett 108:385–389
Harris H (1980) The principles of human biochemical genetics. 3rd ed. Elsevier/North-Holland Press, p 91
Miernyk JA, Dennis DT (1982) Isozymes of the glycolytic enzymes in endosperm from developing castor oil seeds. Plant Physiol 69:825–828
Pichersky E, Gottlieb LD (1983) Evidence for the duplication of the structural genes coding plastid and cytosolic isozymes of triose phosphate isomerase in diploid species of Clarkia. Genetics 105:421–436
Pichersky E, Gottlieb LD (1984) Plant triose phosphate isomerase isozymes: purification, immunological and structural characterization, and partial amino acid sequences. Plant Physiol, in press
Schatz G, Butow RA (1983) How are proteins imported into mitochondria? Cell 32:316–318
Weeden NF, Gottlieb LD (1980) Isolation of cytoplasmic enzymes from pollen. Plant Physiol 66:400–403
Weeden NF, Gottlieb LD (1982) Dissociation, reassociation, and purification of plastid and cytosolic phosphoglucose isomerase isozymes. Plant Physiol 69:717–723
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Communicated by M.M. Green
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Pichersky, E., Gottlieb, L.D. & Higgins, R.C. Hybridization between subunits of triose phosphate isomerase isozymes from different subcellular compartments of higher plants. Molec. Gen. Genet. 193, 158–161 (1984). https://doi.org/10.1007/BF00327430
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DOI: https://doi.org/10.1007/BF00327430