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Comparison of ribosomal proteins of chloroplast from Spinach and of E. coli

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Summary

A comparison of ribosomal proteins from Escherichia coli and from chloroplasts of Spinach was made using two separate methods: electrophoretic migration and immunochemical cross-reaction between blotted E. coli ribosomal proteins and chloroplast ribosomal subunits antisera. It is shown that L2 from E. coli (E-12) and L4 from chloroplasts (CS-L4) comigrated and that E-L4 immunologically cross-reacted with the isolated CS-L4 antibody. Co-migration was observed for three additional couples of 50S ribosomal proteins. It is also shown that at least one 30S E. coli ribosomal protein immuno-cross reacted with a 30S chloroplast antiserum and that three couples of 30S ribosomal proteins comigrated.

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire végétale, Université de Grenoble 1, B.P. 68, F-38402, Saint-Martin d'Hères Cedex, France

    A. M. Dorne, J. Eneas-Filho & R. Mache

  2. Department de Biologie Générale, Université Claude Bernard, Lyon 1, F-69622, Villeurbanne Cedex, France

    P. Heizmann

Authors
  1. A. M. Dorne
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  2. J. Eneas-Filho
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  3. P. Heizmann
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  4. R. Mache
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Communicated by K. Isono

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Dorne, A.M., Eneas-Filho, J., Heizmann, P. et al. Comparison of ribosomal proteins of chloroplast from Spinach and of E. coli . Molec. Gen. Genet. 193, 129–134 (1984). https://doi.org/10.1007/BF00327425

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  • DOI: https://doi.org/10.1007/BF00327425

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