Summary
The swi1 + gene is necessary for effective mating-type (MT) switching in Schizosaccharomyces pombe. It was cloned on a 4.2 kb genomic DNA fragment. By site-directed integration into the genome and gene disruption experiments it was proved that the swi1 + gene itself and not a suppressor had been isolated. Disruption of the swi1 + gene causes a phenotype identical to that of the original swi1 mutant, i.e. the strain still shows some MT switching. The swi1 gene is unique in the genome and gives rise to a 3 kb mRNA.
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Schmidt, H. Strains of Schizosaccharomyces pombe with a disrupted swi1 gene still show some mating-type switching. Mol Gen Genet 210, 485–489 (1987). https://doi.org/10.1007/BF00327201
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DOI: https://doi.org/10.1007/BF00327201