Summary
The recombinant plasmid pIJ3070 isolated from a genomic library of Xanthomonas campestris pv. campestris constructed in the conjugal cosmid pLAFR3 contains protease gene(s) which can be expressed in Escherichia coli. Tn5 mutagenesis and subcloning revealed that the protease structural gene(s) is(are) located in a ca. 10 kb EcoRI fragment. Several protease-minus mutants of X. c. campestris were obtained by Tn5 mutagenesis of pIJ3070 and marker exchange techniques. Studies of pathogenicity of these Tn5 mutants showed that the protease is not critically important for the pathogenicity of X. c. campestris on turnip plants but may play a minor role in disease development.
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Abbreviations
- Gm:
-
gentamicin
- Km:
-
kanamycin
- Rif:
-
rifampicin
- Spc:
-
spectinomycin
- Sm:
-
streptomycin
- Tc:
-
tetracycline
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Tang, J.L., Gough, C.L., Barber, C.E. et al. Molecular cloning of protease gene(s) from Xanthomanas campestris pv. campestris: Expression in Escherichia coli and role in pathogenicity. Mol Gen Genet 210, 443–448 (1987). https://doi.org/10.1007/BF00327195
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DOI: https://doi.org/10.1007/BF00327195