Abstract
Polytene chromosomes of D. melanogaster and D. virilis were hybridized in situ with 125I labeled mRNA isolated from polysomes of D. melanogaster tissue culture cells incubated at 37° C. 125I mRNA hybridized preferentially with subdivisions 87A and 87Cl of the D. melanogaster 3R chromosome; grains were also observed at regions 93D, 95D and over the chromocenter. A considerable cross hybridization of this mRNA with D. virilis polytene chromosomes was observed. The 29C region of the D. virilis second chromosome was the main site of hybridization. Significant grain numbers also appeared in region 20F of the same chromosome. The two regions mentioned belong to heat shock loci in the latter species. Based on label intensity we conclude that region 29C of D. virilis contains DNA sequences retaining molecular homology with those at subdivisions 87A and 87Cl of D. melanogaster. SDS-polyacrylamide gel electrophoresis revealed similar distributions of heat shock proteins in the two species studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Angerer, R.C., Davidson, E.H., Britten, R.J.: Single copy DNA and structural gene sequence relationships among four sea urchin species, Chromosoma (Berl.) 56, 213–226 (1976)
Ashburner, M.: Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments. Chromosoma (Berl.) 31, 356–376 (1970)
Atherton, D.D., Gall, J.G.: Salivary gland squashes for in situ hybridization studies. Dros. Inf. Serv. 49, 131–133 (1972)
Bonner, J.J., Pardue, M.L.: Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Assay by in situ hybridization. Chromosoma (Berl.) 58, 87–99 (1976)
Bonner, W.M., Laskey, R.A.: A film detection method for tritium-labelled proteins and necleic acids in polyacrylamide gels. Europ. J. Biochem. 46, 83–88 (1974)
Breugel, F.M.A., Roy, A., Gloor, H.: A comparison of banding patterns in salivary gland chromosomes of two species of Drosophila. Genetica's-Gravenhage 39, 165–192 (1968)
Bridges, C.B.: Salivary chromosome maps. With a key to the banding of the chromosomes of Drosophila melanogaster. J. Heredity 26, 60–64 (1935)
Brown, D.D., Wensink, P.C., Jordan, E.: A comparison of the ribosomal DNAs of Xenopus laevis and X.mulleri: the evolution of tandem genes. J. molec. Biol. 63, 57–71 (1972)
Cohen, M.: Evolution of 5S ribosomal RNA genes in the chromosome of the virilis group of Drosophila. Chromosoma (Berl.) 55, 359–371 (1976)
Evgen'ev, M.B., Polianskaya, G.G.: The pattern of polytene chromosome synapsis in Drosophila species and interspecific hybrids. Chromosoma (Berl.) 57, 285–295 (1976)
Gall, J.G., Atherton, D.D.: Satellite DNA sequences in Drosophila virilis. J. molec. Biol. 85, 633–664 (1974)
Henikoff, S., Meselson, M.: Transcription at two heat shock loci in Drosophila. Cell 12, 444–451 (1977)
Hennig, W., Hennig, I., Stein, H.: Repeated sequences in the DNA of Drosophila and their localization in giant chromosomes. Chromosoma (Berl.) 32, 31–62 (1970)
Hsu, T.C.: Chromosomal variation and evolution in the virilis group of Drosophila. Univ. Texas Publ. 5204, 35–72 (1952)
Ish-Horowitz, D., Holden, J.J., Gehring, W.L.: Deletion of two heat loci in Drosophila melanogaster and their effects on heat induced protein. Cell 12, 643–652 (1977)
Kakpakov, V.T., Gvozdev, V.A., Platova, T.P., Polukarova, L.G.: The lines of Drosophila melanogaster embryonic cells, maintained in vitro. Genetica (USSR) 5, 67 (1969)
Kikkawa, H.: Studies on the genetics and cytology of Drosophila ananassae. Genetica's-Gravenhage 20, 458–516 (1938)
Laemmli, U.K., Favre, M.: Maturation of the head of bacteriophage T4. J. molec. Biol. 80, 601–613 (1973)
Lambert, B., Beermann, W.: Homology of Balbiani ring DNA in two closely related Chironomus species. Chromosoma (Berl.) 51, 41–47 (1975)
Lefevre, G.: A photographic representation and interpretation of the polytene chromosomes of Drosophila melanogaster salivary glands. In: The genetics and biology of Drosophila. New York: Academic Press 1977
Lewis, M., Helmsing, P.J., Ashburner, M.: Parallel changes in puffing activity and patterns of protein synthesis in salivary glands of Drosophila. Proc. nat. Acad. Sci. (Wash.) 72, 3604–3608 (1975)
Macgregor, H.C., Jones, C.: Chromosomes, DNA sequences and evolution in salamanders of the genus Aneides. Chromosoma (Berl.) 63, 1–10 (1977)
McKenzie S., Henikoff, S., Meselson, M.: Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 72, 1117–1121 (1975)
Pardue, M.L., Kedes, L.H., Weinberg, E.S., Birnstiel, M.L.: Localization of sequences coding for histone messenger RNA in the chromosomes of D. melanogaster. Chromosoma (Berl.) 63, 135–151 (1977)
Patterson, J.T., Stone, W.S.: Evolution in the genus Drosophila. New York: Macmillan Co. 1952
Sokolov, N.N.: Interaction between the nucleus and cytoplasm during distant hybridization of animals. Moscow: Acad. Science USSR Press 1959
Stalker, H.: Intergroup phylogenies in Drosophila as determined by comparisons of salivary banding patterns. Genetics 70, 457–474 (1972)
Tissières, A., Mitchel, H.K., Tracy, U.M.: Protein synthesis in salivary glands of D. melanogaster: relation to chromosome puffs. J. molec. Biol. 84, 389–398 (1974)
Wimber, D.E., Wimber, D.R.: Sites of the 5S ribosomal genes in Drosophila. I. The multiple clusters in the virilis group. Genetics 86, 133–148 (1977)
Wobus, U., Serfling, E. The repetition frequency of DNA in Balbiani Ring 2 of Chironomus thummi. Chromosoma (Berl.) 67, 279–286 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evgen'ev, M.B., Kolchinski, A., Levin, A. et al. Heat-shock DNA homology in distantly related species of Drosophila . Chromosoma 68, 357–365 (1978). https://doi.org/10.1007/BF00327170
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00327170